Gonçalves Luiz Ricardo, Roque André Luiz Rodrigues, Matos Carlos Antonio, Fernandes Simone de Jesus, Olmos Isabella Delamain Fernandez, Machado Rosangela Zacarias, André Marcos Rogério
Pós-graduação em Microbiologia Agropecuária, Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil; Laboratório de Imunoparasitologia, Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil.
Laboratório de Biologia de Tripanosomatídeos, Fundação Oswaldo Cruz/FioCruz, Rio de Janeiro, RJ, Brazil.
Comp Immunol Microbiol Infect Dis. 2015 Dec;43:50-6. doi: 10.1016/j.cimid.2015.10.006. Epub 2015 Oct 28.
Although hemoplasma infection in domestic animals has been well documented, little is known about the prevalence and genetic diversity of these bacteria in wild rodents. The present work aimed to investigate the occurrence of hemotrophic mycoplasmas in wild rodents from five Brazilian biomes, assessing the 16S rRNA phylogenetic position of hemoplasma species by molecular approach. Spleen tissues were obtained from 500 rodents, comprising 52 different rodent species trapped between 2000 and 2011. DNA samples were submitted to previously described PCR protocols for amplifying Mycoplasma spp. based on 16S rRNA, followed by sequencing and phylogenetic inferences. Among 457 rodent spleen samples showing absence of inhibitors, 100 (21.9%) were PCR positive to Mycoplasma spp. The occurrence of hemotropic mycoplasmas among all sampled rodents was demonstrated in all five biomes and ranged from 9.3% (7/75) to 26.2% (38/145). The Blastn analysis showed that amplified sequences had a percentage of identity ranging from 86 to 99% with other murine hemoplasmas. The ML phylogenetic analysis of 16S rRNA gene of 24 positive randomly selected samples showed the presence of ten distinct groups, all clustering within the Mycoplasma haemofelis. The phylogenetic assessment suggests the circulation of novel hemoplasma species in rodents from different biomes in Brazil.
尽管家畜中的血支原体感染已有充分记载，但对于这些细菌在野生啮齿动物中的流行情况和遗传多样性却知之甚少。本研究旨在调查来自巴西五个生物群落的野生啮齿动物中血营养支原体的存在情况，通过分子方法评估血支原体物种的16S rRNA系统发育位置。从500只啮齿动物获取脾脏组织，这些啮齿动物包括2000年至2011年间捕获的52种不同啮齿动物物种。DNA样本按照先前描述的用于基于16S rRNA扩增支原体属的PCR方案进行处理，随后进行测序和系统发育推断。在457份未显示抑制剂的啮齿动物脾脏样本中，100份（21.9%）对支原体属PCR呈阳性。在所有五个生物群落中均证实了所有采样啮齿动物中血营养支原体的存在，其发生率在9.3%（7/75）至26.2%（38/145）之间。Blastn分析表明，扩增序列与其他鼠血支原体的同一性百分比在86%至99%之间。对随机选择的24个阳性样本的16S rRNA基因进行的ML系统发育分析显示存在十个不同的组，所有组均聚类在溶血支原体内。系统发育评估表明巴西不同生物群落的啮齿动物中存在新型血支原体物种。
