Pratt L F, Rassenti L, Larrick J, Robbins B, Banks P M, Kipps T J
Gene Labs, Redwood City, CA.
J Immunol. 1989 Jul 15;143(2):699-705.
Using the polymerase chain reaction we examined for specific Ig kappa-L chain V region gene (V kappa gene) rearrangement in small lymphocytic non-Hodgkin's lymphomas that express Ig bearing a major kappa-L chain associated cross-reactive Id, designated 17.109. Previously, we identified the 17.109-cross-reactive Id in chronic lymphocytic leukemia as a serologic marker for expression of a highly conserved V kappa gene, designated Humkv325. Using sense-strand oligonucleotides specific for the 5'-end of this V kappa gene and antisense oligonucleotide specific for a J kappa region consensus sequence, we could amplify specifically Humkv325 when juxtaposed with J kappa through Ig gene rearrangement. This allowed us to amplify rearranged V kappa genes from DNA isolated from minute amounts of lymphoma biopsy material for molecular analyses. Our studies demonstrate that 17.109-reactive SL NHL, with or without associated CLL, rearrange, and presumably express, Humkv325 without substantial somatic diversification. Our data suggest that malignant B cells in SL NHL, in contrast to NHL of follicular center cell origin, may express immunoglobulin variable region genes with little or no somatic hypermutation.
我们运用聚合酶链反应，检测了表达带有主要κ轻链相关交叉反应性独特型（命名为17.109）的免疫球蛋白的小淋巴细胞性非霍奇金淋巴瘤中特异性免疫球蛋白κ轻链V区基因（Vκ基因）重排情况。此前，我们在慢性淋巴细胞白血病中鉴定出17.109交叉反应性独特型，作为一种高度保守的Vκ基因（命名为Humkv325）表达的血清学标志物。利用针对该Vκ基因5'端的正义链寡核苷酸和针对κ连接区共有序列的反义寡核苷酸，当Humkv325通过免疫球蛋白基因重排与κ连接区并列时，我们能够特异性地扩增它。这使我们能够从微量淋巴瘤活检材料中分离的DNA中扩增重排的Vκ基因，用于分子分析。我们的研究表明，有或没有相关慢性淋巴细胞白血病的17.109反应性小淋巴细胞性非霍奇金淋巴瘤重排并可能表达Humkv325，且没有明显的体细胞多样化。我们的数据表明，与滤泡中心细胞来源的非霍奇金淋巴瘤相反，小淋巴细胞性非霍奇金淋巴瘤中的恶性B细胞可能表达很少或没有体细胞超突变的免疫球蛋白可变区基因。
