Gombart A F, Blissard G W, Rohrmann G F
Department of Agricultural Chemistry, Oregon State University, Corvallis 97331-6502.
J Gen Virol. 1989 Jul;70 ( Pt 7):1815-28. doi: 10.1099/0022-1317-70-7-1815.
A region including the 4 kb HindIII M fragment of the multicapsid nuclear polyhedrosis virus (MNPV) of Orgyia pseudotsugata (OpMNPV) genome was sequenced, transcriptionally mapped, and compared to the homologous region in the MNPV of Autographa californica (AcMNPV). Five open reading frames (ORFs) oriented in the same direction were identified and were found to be expressed at late times post-infection. The mRNAs from the five ORFs were found to coterminate at a single site downstream of ORF 5. The conserved late gene promoter/mRNA start site sequence (AGTAAG) was present upstream of all the ORFs, but did not appear to be the major site of mRNA initiation for two of the ORFs as determined by primer extension analysis. These data indicated that use of this signal for transcriptional initiation may vary between different ORFs. The predicted amino acid sequences for the five ORFs of AcMNPV and OpMNPV were compared and amino acid homologies of 26 to 72% were observed. The comparison revealed a number of major differences in the genomes of the two viruses. A putative transposable element of 634 nucleotides was found to be inserted into the previously reported AcMNPV ORF 1 sequence. In addition, it was found that a region corresponding to the 4 kb HindIII K/EcoRI S/hr5 region of AcMNPV was not present in the OpMNPV genome.
对包括云杉毒蛾多核衣壳核型多角体病毒（OpMNPV）基因组4 kb HindIII M片段的区域进行了测序、转录图谱分析，并与苜蓿银纹夜蛾多核衣壳核型多角体病毒（AcMNPV）的同源区域进行了比较。鉴定出了五个同向的开放阅读框（ORF），并发现它们在感染后晚期表达。发现来自这五个ORF的mRNA在ORF 5下游的单个位点共终止。保守的晚期基因启动子/mRNA起始位点序列（AGTAAG）存在于所有ORF的上游，但通过引物延伸分析确定，对于其中两个ORF而言，它似乎不是mRNA起始的主要位点。这些数据表明，不同ORF之间使用该信号进行转录起始的情况可能有所不同。比较了AcMNPV和OpMNPV五个ORF的预测氨基酸序列，观察到氨基酸同源性为26%至72%。比较揭示了两种病毒基因组中的一些主要差异。发现一个634个核苷酸的推定转座元件插入到先前报道的AcMNPV的ORF 1序列中。此外，还发现OpMNPV基因组中不存在与AcMNPV的4 kb HindIII K/EcoRI S/hr5区域相对应的区域。
