Jin T, Qi Y, Qi B, Jin H, Huang Y
Institute of Virology, Wuhan University, P.R. China.
Virus Genes. 1997;14(3):201-10. doi: 10.1023/a:1007983810311.
A 5423 bp fragment of LsNPV genome was sequenced, in which PDV-E66 gene and another four ORFs were found. The PDV-E66 gene of LsNPV was compared with the PDV-E66 gene of AcNPV, and a 51.9% nucleotide sequence homology and 38.8% amino acid sequence homology were found between the two genes. Two conserved late transcriptional motifs TAAG were found in LsNPV PDV-E66 gene, similar to those in AcNPV PDV-E66. The LsNPV PDV-E66 ORF is 204 base pairs shorter than the AcNPV PDV-E66 ORF at the 5' end. This is agreement with the fact that the N-terminus of the AcNPV PDV-E66 mature protein is 69 amino acids interior to the N-terminus predicted by the AcNPV PDV-E66 ORF. The 5' regulatory region of ORF1 contains early (CGTGC) and late (TAAG) transcriptional initiation motifs and ORF1 is predicted to encode a protein with 114 amino acid residues. The 5' regulatory region of ORF2 which can encode a protein with 115 amino acid residues contains only an early transcriptional initiation motif. Compared with all the genes from AcNPV and other baculoviruses, ORF1 and ORF2 have no homologous genes. It is suggested that ORF1 and ORF2 may be two novel baculovirus genes. ORF3 (PDV-E66 gene), ORF5 and an incomplete ORF, ORF6-part, have homologous regions in the AcNPV genome. ORF3, ORF5, ORF6-part are linked together in LsNPV genome, but their homologous regions are separated by about 58 kb fragment in the AcNPV genome. This fact indicates that the organization of the above genes in LsNPV is different from that of AcNPV. ORF4 is included in ORF6-part and can encode a 48 amino acid residues polypeptide, but ORF4 and ORF6-part are located on different DNA strands.
对LsNPV基因组的一个5423 bp片段进行了测序，发现其中有PDV-E66基因和另外四个开放阅读框（ORF）。将LsNPV的PDV-E66基因与AcNPV的PDV-E66基因进行比较，发现两个基因之间的核苷酸序列同源性为51.9%，氨基酸序列同源性为38.8%。在LsNPV PDV-E66基因中发现了两个保守的晚期转录基序TAAG，与AcNPV PDV-E66中的相似。LsNPV PDV-E66开放阅读框在5'端比AcNPV PDV-E66开放阅读框短204个碱基对。这与AcNPV PDV-E66成熟蛋白的N端比AcNPV PDV-E66开放阅读框预测的N端向内69个氨基酸的事实相符。ORF1的5'调控区包含早期（CGTGC）和晚期（TAAG）转录起始基序，预测ORF1编码一个含有114个氨基酸残基的蛋白质。ORF2的5'调控区可编码一个含有115个氨基酸残基的蛋白质，仅包含一个早期转录起始基序。与AcNPV和其他杆状病毒的所有基因相比，ORF1和ORF2没有同源基因。提示ORF1和ORF2可能是两个新的杆状病毒基因。ORF3（PDV-E66基因）、ORF5和一个不完整的ORF，即ORF6部分，在AcNPV基因组中有同源区域。ORF3、ORF5、ORF6部分在LsNPV基因组中连在一起，但它们的同源区域在AcNPV基因组中被约58 kb的片段隔开。这一事实表明上述基因在LsNPV中的组织方式与AcNPV不同。ORF4包含在ORF6部分中，可编码一个含有48个氨基酸残基的多肽，但ORF4和ORF6部分位于不同的DNA链上。
