微小RNA-223通过调节上皮-间质转化抑制人宫颈癌细胞转移。
MiR-223 inhibited cell metastasis of human cervical cancer by modulating epithelial-mesenchymal transition.
作者信息
Tang Yaling, Wang Yifeng, Chen Qionghua, Qiu Naxuan, Zhao Yan, You Xueye
机构信息
Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University Guangzhou 510282, People's Republic of China ; Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University Xiamen 361003, People's Republic of China.
Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University Guangzhou 510282, People's Republic of China.
出版信息
Int J Clin Exp Pathol. 2015 Sep 1;8(9):11224-9. eCollection 2015.
Abstract
Accumulating evidence have emerged important roles for microRNAs (miRNAs) participating in epithelial-mesenchymal transition (EMT) process and are associated with metastasis in cervical cancer. We hypothesized that miR-223 played an important role in cell metastasis of cervical cancer. Here, we found miR-223 was downregulated in human cervical cancer cell lines and clinical tumor tissues. Result of wound healing and cell migration assays revealed that miR-223 inhibited cell migration, whereas miR-223-in showed the opposite effect. In terms of mechanism, miR-223 influenced the expression of the EMT-associated proteins by upregulating the epithelial markers E-cadherin and α-cadherin and downregulating the mesenchymal marker vimentin. In conclusion, miR-223 inhibited cell metastasis of human cervical cancer by modulating epithelial-mesenchymal transition.
摘要
越来越多的证据表明，微小RNA（miRNA）在参与上皮-间质转化（EMT）过程中发挥着重要作用，并且与宫颈癌转移相关。我们推测miR-223在宫颈癌细胞转移中起重要作用。在此，我们发现miR-223在人宫颈癌细胞系和临床肿瘤组织中表达下调。伤口愈合和细胞迁移实验结果显示，miR-223抑制细胞迁移，而miR-223抑制剂则表现出相反的作用。在机制方面，miR-223通过上调上皮标志物E-钙黏蛋白和α-钙黏蛋白以及下调间质标志物波形蛋白来影响EMT相关蛋白的表达。总之，miR-223通过调节上皮-间质转化抑制人宫颈癌细胞转移。
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J Hepatol. 2015 Oct;63(4):874-85. doi: 10.1016/j.jhep.2015.05.008. Epub 2015 May 18.
2
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IUBMB Life. 2015 May;67(5):380-94. doi: 10.1002/iub.1381. Epub 2015 May 19.
3
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Lab Invest. 2015 Jul;95(7):817-832. doi: 10.1038/labinvest.2015.57. Epub 2015 May 11.
4
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5
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6
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7
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8
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