Department of Animal Biosystem Science, Chungnam National University (CNU) , 99 Daehak-ro, Yusung-gu, Daejeon 34134, Republic of Korea.
Biomedical Translational Research Center, Korea Research Institute of Bioscience and Biotechnology , Daejeon 34141, Republic of Korea.
J Agric Food Chem. 2015 Dec 23;63(50):10803-10. doi: 10.1021/acs.jafc.5b04790. Epub 2015 Dec 11.
α-Lactalbumin and β-lactoglobulin are two major whey proteins that specifically bind immunoglobulin E and are suspected as major allergens causing cow's milk allergy (CMA). Recent studies have shown that thymic stromal lymphopoietin is a critical factor linking at the interface of the body and environment to the T-helper 2 response. However, it is not known whether thymic stromal lymphopoietin expression is changed by α-lactalbumin and β-lactoglobulin in immune cells. Using RT-PCR and ELISA, the present study was conducted to examine if intravenous injection of α-lactalbumin and β-lactoglobulin increased pro-inflammatory cytokines, T-helper 2 cytokines, and thymic stromal lymphopoietin expression in several immune cells, including macrophages, mast cells, and keratinocytes. Results showed that α-lactalbumin and β-lactoglobulin induced thymic stromal lymphopoietin, interleukin-6, and tumor necrosis factor-α expression. It was concluded that the allergenicity of α-lactalbumin and β-lactoglobulin may be attributed to thymic stromal lymphopoietin induction, T-helper 2 cytokines, and pro-inflammatory cytokines.
α-乳白蛋白和β-乳球蛋白是两种主要的乳清蛋白，它们特异性地结合免疫球蛋白 E，被怀疑是引起牛奶过敏（CMA）的主要过敏原。最近的研究表明，胸腺基质淋巴细胞生成素是将身体和环境之间的界面与辅助性 T 细胞 2 反应联系起来的关键因素。然而，目前尚不清楚α-乳白蛋白和β-乳球蛋白是否会改变免疫细胞中的胸腺基质淋巴细胞生成素表达。本研究采用 RT-PCR 和 ELISA 法，研究静脉注射α-乳白蛋白和β-乳球蛋白是否会增加几种免疫细胞（包括巨噬细胞、肥大细胞和角质形成细胞）中促炎细胞因子、辅助性 T 细胞 2 细胞因子和胸腺基质淋巴细胞生成素的表达。结果表明，α-乳白蛋白和β-乳球蛋白诱导了胸腺基质淋巴细胞生成素、白细胞介素-6 和肿瘤坏死因子-α 的表达。结论认为，α-乳白蛋白和β-乳球蛋白的变应原性可能归因于胸腺基质淋巴细胞生成素诱导、辅助性 T 细胞 2 细胞因子和促炎细胞因子。
