选择性散射光谱作为研究基粒和基质叶绿体内部结构的一种方法。
Selective scattering spectra as an approach to internal structure of granal and agranal chloroplasts.
作者信息
Bialek G E, Horváth G, Garab G I, Mustárdy L A, Faludi-Dániel A
出版信息
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1455-7. doi: 10.1073/pnas.74.4.1455.
Abstract
Selective scattering spectra of granal and agranal chloroplasts were measured in the red spectral region and compared with calculations based on the Mie theory. The spectra were influenced considerably by the intactness and ultrastructural pattern of the chloroplasts. It was demonstrated that the spectra consist of two components: one attributable to grana and the other, to single lamellae. The dependence of the selective scattering spectra on the ultrastructural characteristics offers a convenient method for monitoring the quality of chloroplast preparations by a procedure much faster than electron microscopy.
摘要
在红色光谱区域测量了基粒叶绿体和基质叶绿体的选择性散射光谱，并与基于米氏理论的计算结果进行了比较。这些光谱受叶绿体的完整性和超微结构模式的影响很大。结果表明，这些光谱由两个成分组成：一个归因于基粒，另一个归因于单个片层。选择性散射光谱对超微结构特征的依赖性提供了一种比电子显微镜更快的方法来监测叶绿体制剂的质量。
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本文引用的文献
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