L细胞分泌的神经生长因子的分子特性
Molecular properties of the nerve growth factor secreted by L cells.
作者信息
Pantazis N J, Blanchard M H, Arnason B G, Young M
出版信息
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1492-6. doi: 10.1073/pnas.74.4.1492.
Abstract
The molecular size and stability of the nerve growth factor (NGF) secreted in culture by L cells have been studied by sedimentation and gel filtration chromatography. Results indicate that L cell NGF has a molecular weight of 160,000. It contains as part of its structure a protein component that is biologically, immunologically, and electrophoretically indistinguishable from the biologically active factor purified from mouse submandibular glands. However, unlike pure gland NGF, L cell NGF is highly stable in solution, and this finding indicates that L cell NGF is a form of the factor different from that previously described.
摘要
通过沉降和凝胶过滤色谱法研究了L细胞在培养物中分泌的神经生长因子（NGF）的分子大小和稳定性。结果表明，L细胞NGF的分子量为160,000。其结构的一部分包含一种蛋白质成分，该成分在生物学、免疫学和电泳方面与从小鼠下颌下腺纯化的生物活性因子无法区分。然而，与纯腺体NGF不同，L细胞NGF在溶液中高度稳定，这一发现表明L细胞NGF是一种与先前描述的因子不同的形式。
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本文引用的文献
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