Pang Xiaowen, Zhang Ping, Zhu Sainan, Guo Guangjin
Department of Dermatology, Air Force General Hospital of People's Liberation Army, Beijing 100142, P.R. China.
Department of Statistics, The First Hospital of Peking University, Beijing 100030, P.R. China.
Exp Ther Med. 2015 Aug;10(2):419-422. doi: 10.3892/etm.2015.2522. Epub 2015 May 26.
Expression of glucocorticoid receptor (GR)-α is observed in almost all tissues and cells of the body; thus, investigating the expression of the receptor in the epidermis of patients with psoriasis vulgaris. The aim of the present study was to investigate GR-α expression in the epidermis of psoriasis vulgaris patients The study population consisted of 26 patients with psoriasis vulgaris and 10 normal control cases. None of the patients had received any prior treatment with glucocorticoids. Epithelial tissue samples were detected using a streptavidin peroxidation-enzymatic method for biopsy. Non-lesional tissue samples from within a range of 3 cm of lesions formed the non-lesional group, and lesional tissue samples formed the lesional group. Pathological image analysis system (named CMIAS) was used to convert image signals into numeric values, according to the optical density per unit area. GR-α expression was observed within the nucleus in the normal control group; however, cytoplasmic expression was observed in the lesions of the psoriatic group. The optical density values were significantly lower in the psoriatic group when compared with the normal control group, indicating a statistically significant difference in GR-α expression between the two groups (P<0.001), and this decreasing correlation was unaffected by the administration of steroids for 6 months. Therefore, decreased expression of GR-α may play an important role in the degeneration of keratinocytes in patients with psoriasis vulgaris.
糖皮质激素受体（GR）-α在人体几乎所有组织和细胞中均有表达；因此，研究寻常型银屑病患者表皮中该受体的表达情况。本研究的目的是调查寻常型银屑病患者表皮中GR-α的表达。研究对象包括26例寻常型银屑病患者和10例正常对照者。所有患者均未接受过糖皮质激素的任何前期治疗。采用链霉亲和素过氧化酶法对活检的上皮组织样本进行检测。距离皮损3 cm范围内的非皮损组织样本构成非皮损组，皮损组织样本构成皮损组。使用病理图像分析系统（名为CMIAS）根据单位面积的光密度将图像信号转换为数值。在正常对照组中观察到GR-α在细胞核内表达；然而，在银屑病组的皮损中观察到细胞质表达。与正常对照组相比，银屑病组的光密度值显著降低，表明两组之间GR-α表达存在统计学显著差异（P<0.001），且这种下降相关性不受6个月类固醇给药的影响。因此，GR-α表达降低可能在寻常型银屑病患者角质形成细胞退变中起重要作用。
