人细小病毒B19的分子特征
Molecular characterization of human Torque Teno virus.
作者信息
Wei Youping, Chen Minyang, Yang Xia, Zhang Liming, Rao Lihua, Yuan Feifang, Wang Yangqin, Gong Jing, Li Lianping
机构信息
Nanchang Hospital of Integrated Traditional Chinese and Western Medicine, Nanchang, Jiangxi 330003, P.R. China.
出版信息
Biomed Rep. 2015 Nov;3(6):821-826. doi: 10.3892/br.2015.508. Epub 2015 Aug 26.
The present study analyzed the presence of human Torque Teno virus (TTV) in hospitalized patients from different departments. In total, 378 serum specimens were collected from the patients (171 with cardiovascular disease, 192 with tumor and 15 with gastroenteritis) and analyzed by ELISA and nest-polymerase chain reaction (PCR) to detect the presence of TTV. The results showed that 64 specimens (17%) were TTV positive from detection with the human ELISA kit, and the patients aged <30 years have a higher prevalence. TTV in males was more common than in female patients. In addition, nest-PCR was used to detect TTV within different phylogenetic groups among the 64 specimens, and the results showed that groups 1 (TA278 strain), 4 (KC009) and 5 (CT39) were much more prevalent than groups 2 (PMV isolate) and 3 (11 genotypes) in the different departmental patients.
本研究分析了不同科室住院患者中人类细小病毒B19（TTV）的存在情况。总共从患者中收集了378份血清样本（171例心血管疾病患者、192例肿瘤患者和15例肠胃炎患者），并通过酶联免疫吸附测定（ELISA）和巢式聚合酶链反应（PCR）分析以检测TTV的存在。结果显示，用人ELISA试剂盒检测出64份样本（17%）呈TTV阳性，年龄<30岁的患者患病率更高。TTV在男性中比在女性患者中更常见。此外，采用巢式PCR检测64份样本中不同系统发育组内的TTV，结果显示，在不同科室的患者中，第1组（TA278株）、第4组（KC009）和第5组（CT39）比第2组（PMV分离株）和第3组（11种基因型）更为普遍。
Zotero 插件