Siahpoush Mohammad, Noorbazargan Hassan, Kalantari Shirin, Shayestehpour Mohammad, Yazdani Shaghayegh
Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Microbiol. 2022 Apr;14(2):181-185. doi: 10.18502/ijm.v14i2.9185.
Torque Teno virus or transfusion-transmitted virus (TTV) is a non-enveloped virus with a single strand circular DNA genome that currently is classified in the Alphatorquevirus genus and the family of . Unlike other DNA viruses, TTV has an extremely wide genomic diversity. This virus, based on previous studies, infects both healthy people, as well as those who have HCV and human papillomavirus (HPV). This study aimed to evaluate the coinfection of torque teno virus (TTV) and HPV in cervical samples from Iranian women.
In this case-control study, the fresh cervical cytobrush specimens were collected from 150 women referred to Dena laboratory in Tehran. Viral DNA was extracted from samples. The HPV-DNA was detected and genotyped. Then, nested polymerase chain reaction (Nested PCR) was performed for TTV using specific primers.
Among 50 cervical specimens without HPV, 14 were TTV positive (28%); among 50 low-risk HPV cervical specimen, 23 were TTV positive (46%), and from 50 high-risk HPV cervical specimen, 48 were TTV positive (96%). There is a significantly higher prevalence of TTV virus in low-risk and high-risk papillomavirus-infected specimens than in healthy specimens (p 0.0001). Additionally, TTV is more prevalent in samples containing high-risk papillomaviruses than in samples with low-risk papillomaviruses (P = 0.048).
The higher prevalence of TTV among people infected with papillomavirus than in non-infected people indicates that both viruses are transmitted by the same mechanism (sexual route). In addition, the prevalence of TTV in samples containing high-risk papillomavirus is significantly higher than that in samples containing low-risk papillomavirus. The presence of papillomaviruses, particularly high-risk types, may be associated with TTV proliferation, which requires further research in the future.
细小病毒Torque Teno病毒（Torque Teno virus，TTV）或输血传播病毒（transfusion - transmitted virus，TTV）是一种无包膜病毒，其基因组为单链环状DNA，目前被归类于细小病毒属（Alphatorquevirus）及圆环病毒科（Circoviridae）。与其他DNA病毒不同，TTV具有极其广泛的基因组多样性。根据以往研究，该病毒可感染健康人群，以及丙型肝炎病毒（HCV）和人乳头瘤病毒（HPV）感染者。本研究旨在评估伊朗女性宫颈样本中细小病毒Torque Teno病毒（TTV）与HPV的合并感染情况。
在这项病例对照研究中，从转诊至德黑兰Dena实验室的150名女性中收集新鲜宫颈细胞刷标本。从样本中提取病毒DNA。检测HPV - DNA并进行基因分型。然后，使用特异性引物对TTV进行巢式聚合酶链反应（Nested PCR）。
在50份无HPV的宫颈标本中，14份TTV呈阳性（28%）；在50份低风险HPV宫颈标本中，23份TTV呈阳性（46%），而在50份高风险HPV宫颈标本中，48份TTV呈阳性（96%）。低风险和高风险乳头瘤病毒感染标本中TTV病毒的患病率显著高于健康标本（p < 0.0001）。此外，TTV在含有高风险乳头瘤病毒的样本中比在含有低风险乳头瘤病毒的样本中更普遍（P = 0.048）。
乳头瘤病毒感染者中TTV的患病率高于未感染者，这表明两种病毒通过相同机制（性传播途径）传播。此外，含有高风险乳头瘤病毒的样本中TTV的患病率显著高于含有低风险乳头瘤病毒的样本。乳头瘤病毒的存在，尤其是高风险类型，可能与TTV增殖有关，这在未来需要进一步研究。
