Malik A, Wahid H, Rosnina Y, Kasim A, Sabri M
Department of Animal Science, Faculty of Agriculture, Islamic Kalimantan University, Banjarmasin, Indonesia ; Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.
Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.
Open Vet J. 2012;2(1):1-5. Epub 2012 Jan 18.
The objectives of this study were to evaluate estrus response and pregnancy rates resulting from timed artificial insemination (AI) following estrus synchronization using CIDR in postpartum beef cattle. A total of 100 cows were randomly divided into three groups. Groups 1, 2 and 3 were artificially inseminated at 48-50 h (n=30), 53-55 h (n=30) and 58-60 h (n=40) after CIDR removal, respectively. Estrus synchronization was carried out using a CIDR containing 1.38 mg progesterone. All cows were given 2 mg estradiol benzoate, intramuscularly on the day of CIDR insertion (D 0). The CIDR was removed after 8 days and 125 μg of prostaglandin F2α (PGF2α) was injected intramuscularly. One day after CIDR removal all cows were given 1 mg of estradiol benzoate intramuscularly (D 9). Cows were observed visually for estrus after removal of CIDR. Between 30 and 32 days after timed AI, pregnancy was determined using transrectal ultrasonography. The first estrus observation which is approximately 32 h after CIDR removal showed no significant difference (P>0.05) among the three groups. The onset response of estrus after 32 h removal of CIDR was less than 10% in all three groups 6.6% (G1), 6.8% (G2) and 7.3% (G3). Furthermore, percentages of estrus response (D 10) following CIDR removal were 76.6%, 75.0% and 77.5%. The difference between on D 9 and D 10 estrus response were statistically significant (P<0.05). The pregnancy rates were 23.3% (G1), 26.6% (G2) and 37.5% (G3), which were not significant (P>0.05).
本研究的目的是评估产后肉牛使用阴道孕酮释放环（CIDR）进行发情同步后定时人工授精（AI）的发情反应和妊娠率。总共100头母牛被随机分为三组。第1组、第2组和第3组分别在取出CIDR后48 - 50小时（n = 30）、53 - 55小时（n = 30）和58 - 60小时（n = 40）进行人工授精。使用含1.38毫克孕酮的CIDR进行发情同步。所有母牛在插入CIDR当天（第0天）肌肉注射2毫克苯甲酸雌二醇。8天后取出CIDR，并肌肉注射125微克前列腺素F2α（PGF2α）。取出CIDR后一天，所有母牛肌肉注射1毫克苯甲酸雌二醇（第9天）。取出CIDR后对母牛进行发情的肉眼观察。在定时人工授精后30至32天，通过经直肠超声检查确定妊娠情况。首次发情观察约在取出CIDR后32小时，三组之间无显著差异（P>0.05）。在取出CIDR 32小时后，所有三组的发情起始反应均低于10%，分别为6.6%（第1组）、6.8%（第2组）和7.3%（第3组）。此外，取出CIDR后的发情反应百分比（第10天）分别为76.6%、75.0%和77.5%。第9天和第10天发情反应的差异具有统计学意义（P<0.05）。妊娠率分别为23.3%（第1组）、26.6%（第2组）和37.5%（第3组），差异不显著（P>0.05）。
