Fang Lijing, Pan Zhengyin, Cullis Paul M, Burley Glenn A, Su Wu
Guangdong Key Laboratory of Nanomedicine, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, People's Republic of China.
Department of Chemistry, University of Leicester, Leicester, United Kingdom.
Curr Protoc Nucleic Acid Chem. 2015 Dec 1;63:8.11.1-8.11.14. doi: 10.1002/0471142700.nc0811s63.
In this unit, the fully automated solid-phase synthetic strategy of hairpin Py-Im polyamides is described using triphosgene (BTC) as a coupling agent. This automated methodology is compatible with all the typical building blocks, enabling the facile synthesis of polyamide libraries in 9% to 20% yield in 3 days.
在本单元中，描述了以三光气（BTC）作为偶联剂的发夹型Py-Im聚酰胺的全自动固相合成策略。这种自动化方法与所有典型的结构单元兼容，能够在3天内以9%至20%的产率轻松合成聚酰胺文库。
