Franjević Damjan, Novosel Maja, Koletić Nikola
Department of Biology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, Croatia.; Email:
Department of Biology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, Croatia.; Email: unknown.
Zootaxa. 2015 Oct 15;4032(2):221-8. doi: 10.11646/zootaxa.4032.2.9.
Freshwater and brackish species of bryozoans belong to the Phylactolaemata and Gymnolaemata class. Twelve species of bryozoans were recorded and morphologically determined at eight locations in the Black Sea and the Adriatic basin in Croatia. Twelve species of Bryozoa have been listed in the taxonomic index for Croatia (Conopeum seurati, Lophopus crystallinus Paludicella articulata, Cristatella mucedo, Fredericella sultana, Hyalinella punctata, Plumatella casmiana, Plumatella emarginata, Plumatella fruticosa, Plumatella fungosa, Plumatella geimermassardi and Plumatella repens). For the purposes of gene identification of recorded species, molecular markers for nuclear 18S and 28S genes, ITS2 region and mitochondrial COI gene were amplified. Genetic identifications of morphologically determined bryozoan species were confirmed using highly similar sequences local alignment analysis. Proliferation of freshwater bryozoan species over long distances with the help of the vector animals was confirmed by defining haplotypes on the base of 18S, 28S and ITS2 sequences associated with the Black Sea-Mediterranean waterfowl flyway.
淡水和咸淡水苔藓虫物种属于被唇纲和裸唇纲。在克罗地亚的黑海和亚得里亚海盆地的八个地点记录并从形态上确定了12种苔藓虫。克罗地亚的分类索引中列出了12种苔藓虫（苏氏苔藓虫、晶苔藓虫、节苔藓虫、苔虫、苏氏弗雷德里克苔藓虫、点斑透明苔藓虫、里海羽苔虫、具缘羽苔虫、丛生羽苔虫、真菌羽苔虫、盖氏羽苔虫和匐枝羽苔虫）。为了对记录的物种进行基因鉴定，扩增了核18S和28S基因、ITS2区域以及线粒体COI基因的分子标记。通过高度相似序列的局部比对分析，证实了形态学确定的苔藓虫物种的基因鉴定。通过基于与黑海 - 地中海水鸟迁徙路线相关的18S、28S和ITS2序列定义单倍型，证实了淡水苔藓虫物种借助载体动物进行长距离扩散。
