用于检测种牛中胎儿三毛滴虫抗原的基于多克隆抗体的酶免疫测定法的开发与初步评估

Development and preliminary assessment of a polyclonal antibody-based enzyme immunoassay for the detection of Tritrichomonas foetus antigen in breeding cattle.

作者信息

Yule A, Skirrow S Z, Staats J, Bondurant R H

机构信息

Department of Medical Protozoology, London School of Hygiene and Tropical Medicine, Gt. Britain.

出版信息

Vet Parasitol. 1989 May;31(2):115-23. doi: 10.1016/0304-4017(89)90026-5.

DOI:10.1016/0304-4017(89)90026-5

PMID:2662571

Abstract

More sensitive tests are required for the diagnosis of Tritrichomonas foetus infection in cattle and an antigen-detecting enzyme immunoassay (EIA) has been applied to this purpose. An affinity purified immunoglobulin fraction obtained from rabbits immunised with cultured T. foetus served as both capture antibody and as biotinylated indicator antibody. While highly sensitive in the detection of antigen derived from cultured organisms, the assay showed poor sensitivity in the detection of antigen in the cervico-vaginal mucus of artificially infected heifers, with only 75% of culture-positive samples being considered positive for antigen. In a direct comparison, 23/122 samples from a naturally infected dairy herd gave positive cultures, while only 10/122 samples were considered antigen positive by EIA.

摘要

牛胎儿三毛滴虫感染的诊断需要更灵敏的检测方法，为此已应用了一种抗原检测酶免疫测定法（EIA）。从用培养的胎儿三毛滴虫免疫的兔子中获得的亲和纯化免疫球蛋白组分用作捕获抗体和生物素化指示抗体。该检测方法在检测培养生物体来源的抗原时具有高灵敏度，但在检测人工感染小母牛的宫颈-阴道黏液中的抗原时灵敏度较差，只有75%培养阳性的样本被认为抗原检测呈阳性。在直接比较中，来自自然感染奶牛群的122份样本中有23份培养呈阳性，而酶免疫测定法仅将其中10份样本判定为抗原阳性。