Li Xiao-Tong, Yu Qi, Zhou Qi-Sheng, Zhao Xiao, Liu Zhao-Yang, Cui Wei-Zheng, Liu Qing-Xin
Laboratory of Developmental Genetics, Shandong Agricultural University, Tai'an, Shandong 271018, China.
Laboratory of Developmental Genetics, Shandong Agricultural University, Tai'an, Shandong 271018, China.
Gene. 2016 Feb 15;577(2):174-9. doi: 10.1016/j.gene.2015.11.035. Epub 2015 Nov 25.
Axon guidance is critical for proper wiring of the nervous system. During the neural development, the axon guidance molecules play a key role and direct axons to choose the correct way to reach the target. Robo, as the receptor of axon guidance molecule Slit, is evolutionarily conserved from planarians to humans. However, the function of Robo in the silkworm, Bombyx mori, remained unknown. In this study, we cloned robo2/3 from B. mori (Bmrobo2/3), a homologue of robo2/3 in Tribolium castaneum. Moreover, BmRobo2/3 was localized in the neuropil, and RNAi-mediated knockdown of Bmrobo2/3 resulted in the longitudinal connectives forming closer to the midline. These data demonstrate that BmRobo2/3 is required for axon guidance in the silkworm.
轴突导向对于神经系统的正确布线至关重要。在神经发育过程中，轴突导向分子起着关键作用，并引导轴突选择正确的路径到达目标。Robo作为轴突导向分子Slit的受体，从涡虫到人类在进化上是保守的。然而，Robo在家蚕中的功能仍然未知。在本研究中，我们从家蚕中克隆了robo2/3（Bmrobo2/3），它是赤拟谷盗中robo2/3的同源物。此外，BmRobo2/3定位于神经纤维网，RNA干扰介导的Bmrobo2/3敲低导致纵向神经索向中线形成得更近。这些数据表明BmRobo2/3是家蚕轴突导向所必需的。
