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中国亚热带南岭国家级自然保护区森林酸性土壤中氨氧化古菌和细菌的差异分布模式

Differential distribution patterns of ammonia-oxidizing archaea and bacteria in acidic soils of Nanling National Nature Reserve forests in subtropical China.

作者信息

Gan Xian-Hua, Zhang Fang-Qiu, Gu Ji-Dong, Guo Yue-Dong, Li Zhao-Qing, Zhang Wei-Qiang, Xu Xiu-Yu, Zhou Yi, Wen Xiao-Ying, Xie Guo-Guang, Wang Yong-Feng

机构信息

Guangdong Provincial Key Laboratory of Bio-control for the Forest Disease and Pest, Guangdong Academy of Forestry, No. 233, Guangshan 1st Road, Guangzhou, People's Republic of China.

Laboratory of Microbial Ecology and Toxicology, Guangdong Academy of Forestry, No. 233, Guangshan 1st Road, Guangzhou, People's Republic of China.

出版信息

Antonie Van Leeuwenhoek. 2016 Feb;109(2):237-51. doi: 10.1007/s10482-015-0627-8. Epub 2015 Dec 1.

Abstract

In addition to ammonia-oxidizing bacteria (AOB) the more recently discovered ammonia-oxidizing archaea (AOA) can also oxidize ammonia, but little is known about AOA community structure and abundance in subtropical forest soils. In this study, both AOA and AOB were investigated with molecular techniques in eight types of forests at surface soils (0-2 cm) and deep layers (18-20 cm) in Nanling National Nature Reserve in subtropical China. The results showed that the forest soils, all acidic (pH 4.24-5.10), harbored a wide range of AOA phylotypes, including the genera Nitrosotalea, Nitrososphaera, and another 6 clusters, one of which was reported for the first time. For AOB, only members of Nitrosospira were retrieved. Moreover, the abundance of the ammonia monooxygenase gene (amoA) from AOA dominated over AOB in most soil samples (13/16). Soil depth, rather than forest type, was an important factor shaping the community structure of AOA and AOB. The distribution patterns of AOA and AOB in soil layers were reversed: AOA diversity and abundances in the deep layers were higher than those in the surface layers; on the contrary, AOB diversity and abundances in the deep layers were lower than those in the surface layers. Interestingly, the diversity of AOA was positively correlated with pH, but negatively correlated with organic carbon, total nitrogen and total phosphorus, and the abundance of AOA was negatively correlated with available phosphorus. Our results demonstrated that AOA and AOB were differentially distributed in acidic soils in subtropical forests and affected differently by soil characteristics.

摘要

除了氨氧化细菌(AOB)外,最近发现的氨氧化古菌(AOA)也能氧化氨,但对于亚热带森林土壤中AOA的群落结构和丰度了解甚少。在本研究中,利用分子技术对中国亚热带南岭国家级自然保护区8种森林表层土壤(0 - 2厘米)和深层土壤(18 - 20厘米)中的AOA和AOB进行了调查。结果表明,所有森林土壤均呈酸性(pH 4.24 - 5.10),拥有广泛的AOA系统发育型,包括亚硝化螺菌属、亚硝化球形菌属以及另外6个簇,其中一个是首次报道。对于AOB,仅检索到亚硝化螺菌属的成员。此外,在大多数土壤样本(13/16)中,来自AOA的氨单加氧酶基因(amoA)丰度高于AOB。土壤深度而非森林类型是塑造AOA和AOB群落结构的重要因素。AOA和AOB在土壤层中的分布模式相反:深层土壤中AOA的多样性和丰度高于表层土壤;相反,深层土壤中AOB的多样性和丰度低于表层土壤。有趣的是,AOA的多样性与pH呈正相关,但与有机碳、总氮和总磷呈负相关,而AOA的丰度与有效磷呈负相关。我们的结果表明,AOA和AOB在亚热带森林酸性土壤中分布不同,且受土壤特性的影响也不同。

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