Zhang Jingxu, Dai Yu, Wang Yilin, Wu Zhen, Xie Shuguang, Liu Yong
State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China.
Appl Microbiol Biotechnol. 2015 Aug;99(16):6899-909. doi: 10.1007/s00253-015-6625-y. Epub 2015 May 7.
Ammonia oxidation is known to be performed by both ammonia-oxidizing archaea (AOA) and bacteria (AOB), although their relative significance to nitrification process in soil ecosystems remains controversial. The distribution of AOA and AOB in plateau soils with different land use types and the influential factors remains unclear. The present study investigated the abundance and structure of AOA and AOB communities in upland soils adjacent to Erhai Lake in the Yunnan Plateau (China). Quantitative PCR assays indicated a large variation in the community size of AOA and AOB communities, with the numerical dominance of AOA over AOB in most of soils. Clone library analysis illustrated a marked shift in the structure of soil AOA and AOB communities. A high abundance of Nitrososphaera- and Nitrosotalea-like AOA was observed, while Nitrosospira-like species predominated in AOB. AOA and AOB abundance was positively influenced by total nitrogen and moisture content, respectively. Moreover, moisture content might be a key determinant of AOA community composition, while C/N and nitrate nitrogen played an important role in shaping AOB community composition. However, further efforts will be necessary in order to elucidate the links between soil AOA and AOB and land use.
已知氨氧化作用由氨氧化古菌（AOA）和细菌（AOB）共同完成，尽管它们在土壤生态系统硝化过程中的相对重要性仍存在争议。不同土地利用类型的高原土壤中AOA和AOB的分布及其影响因素尚不清楚。本研究调查了中国云南高原洱海附近旱地土壤中AOA和AOB群落的丰度和结构。定量PCR分析表明，AOA和AOB群落的大小存在很大差异，在大多数土壤中AOA在数量上占主导地位。克隆文库分析表明土壤AOA和AOB群落结构发生了显著变化。观察到高丰度的类硝化球菌属和类亚硝化螺菌属AOA，而AOB中类亚硝化螺菌属物种占主导地位。AOA和AOB的丰度分别受到总氮和水分含量的正向影响。此外，水分含量可能是AOA群落组成的关键决定因素，而碳氮比和硝态氮在塑造AOB群落组成方面发挥着重要作用。然而，为了阐明土壤AOA和AOB与土地利用之间的联系，还需要进一步努力。
