大肠杆菌延伸因子Tu-鸟苷5'-三磷酸类似物的制备。
Preparation of Escherichia coli elongation factor Tu-guanosine 5'-triphosphate analogs.
作者信息
Delaria K, Jurnak F
机构信息
Department of Biochemistry, University of California, Riverside 92521.
出版信息
Anal Biochem. 1989 Feb 15;177(1):188-93. doi: 10.1016/0003-2697(89)90038-9.
PMID:2662816
Abstract
A simple procedure for the bulk preparation of 20 mg of Escherichia coli elongation factor (EF)-Tu-GTP analogs is described. The protocol is based upon the preparation and stabilization of nucleotide-free EF-Tu using an EF-Ts affinity chromatographic resin. The procedure is a general one for the preparation of any GTP analog of EF-Tu.
摘要
描述了一种批量制备20毫克大肠杆菌延伸因子（EF）-Tu-GTP类似物的简单方法。该方案基于使用EF-Ts亲和色谱树脂制备和稳定无核苷酸的EF-Tu。该方法是制备EF-Tu任何GTP类似物的通用方法。
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