Gao Shegan, Zhang Mengxi, Zhu Xiaojuan, Qu Zhifeng, Shan Tanyou, Xie Xuanhu, Wang Ying, Feng Xiaoshan
Department of Oncology, The First Affiliated Hospital of Henan University of Science and Technology No. 24 Jinghua Road, Luoyang, Henan, China.
Int J Clin Exp Med. 2015 Sep 15;8(9):15098-107. eCollection 2015.
Photodynamic therapy (PDT) using photofrin-II is a clinically effective treatment for both non-neoplastic and neoplastic diseases. Herein, we performed an in vitro experiment to study the anti-tumor effect and mechanisms of photofrin-II mediated PDT for esophageal squamous cell carcinoma (ESCC) cell line, SHEEC. In this study, human ESCC cell line SHEEC and parental normal cell line SHEE were used. The anti-tumor effect of PDT was determined by evaluating cell viability using CCK-8 assay, apoptosis and generation of reactive oxygen species (ROS). PDT induced significant apoptosis in SHEEC and SHEE cells in a time- and photofrin-II dose-dependent manner. Furthermore, PDT treatment induced significant death of SHEEC, instead of SHEE cells. The apoptotic outcome was accompanied by concurrent generation of ROS. In summary, PDT shed light on therapy of ESCC, functioning as a useful tool for ESCC clinical treatment, providing a better understanding of Photofrin-Diomed 630-PDT in SHEEC cells.
使用二血卟啉醚的光动力疗法（PDT）是一种对非肿瘤性和肿瘤性疾病均具有临床疗效的治疗方法。在此，我们进行了一项体外实验，以研究二血卟啉醚介导的PDT对食管鳞状细胞癌（ESCC）细胞系SHEEC的抗肿瘤作用及其机制。在本研究中，使用了人ESCC细胞系SHEEC和亲本正常细胞系SHEE。通过CCK-8法评估细胞活力、细胞凋亡以及活性氧（ROS）的生成来确定PDT的抗肿瘤作用。PDT以时间和二血卟啉醚剂量依赖性方式诱导SHEEC和SHEE细胞发生显著凋亡。此外，PDT处理导致SHEEC细胞显著死亡，而SHEE细胞未出现这种情况。凋亡结果伴随着ROS的同时生成。总之，PDT为ESCC的治疗提供了新的思路，是ESCC临床治疗的一种有效手段，有助于更好地理解二血卟啉醚-迪奥美630-PDT在SHEEC细胞中的作用。
