Zhang Feng, Zhu Fang-Bing, Li Jia-Jia, Zhang Ping-Ping, Zhu Jun-Feng
Department of Hematology, The First Affiliated Hospital of Bengbu Medical College Bengbu 233004, Anhui, P. R. China.
Int J Clin Exp Med. 2015 Sep 15;8(9):15290-5. eCollection 2015.
Hyperoside (Hyp) is the chief component of some Chinese herbs which has anticancer effect and the present study is to identify whether it could enhance the anti leukemic properties of arsenic trioxide (As2O3) in acute myeloid leukemia (AML). We provide evidence on the concomitant treatment of HL-60 human AML cells with hyperoside potentiates As2O3-dependent induction of apoptosis. The activation of caspase-9, Bcl-2-associated agonist of cell death (BAD), p-BAD, p27 was assessed by Western blot. Results showed that hyperoside inhibited BAD from phosphorylating, reactivated caspase-9, and increased p27 levels. Importantly, hyperoside demonstrated its induction of autophagy effect by upregulation of LC-II in HL-60 AML cell line. Taken together, hyperoside may serve as a great candidate of concomitant treatment for leukemia; these effects were probably related to induction of autophagy and enhancing apoptosis-inducing action of As2O3.
金丝桃苷（Hyp）是一些具有抗癌作用的中草药的主要成分，本研究旨在确定其是否能增强三氧化二砷（As2O3）对急性髓系白血病（AML）的抗白血病特性。我们提供的证据表明，金丝桃苷与HL-60人AML细胞同时处理可增强As2O3依赖性的细胞凋亡诱导作用。通过蛋白质印迹法评估半胱天冬酶-9、细胞死亡的Bcl-2相关激动剂（BAD）、磷酸化BAD（p-BAD）、p27的激活情况。结果表明，金丝桃苷抑制BAD磷酸化，重新激活半胱天冬酶-9，并提高p27水平。重要的是，金丝桃苷通过上调HL-60 AML细胞系中的LC-II来证明其诱导自噬的作用。综上所述，金丝桃苷可能是白血病联合治疗的理想候选药物；这些作用可能与诱导自噬和增强As2O3的凋亡诱导作用有关。
