过表达VEGFl65的血管内皮祖细胞移植可减轻深静脉血栓切除术后的内皮损伤。
Transplantation of VEGFl65-overexpressing vascular endothelial progenitor cells relieves endothelial injury after deep vein thrombectomy.
作者信息
Tang Jun-Jian, Meng Qing-You, Cai Zhi-Xin, Li Xiao-Qiang
机构信息
Department of Vascular Surgery, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China; Department of Vascular Surgery, Taihu Hospital, Wuxi, Jiangsu, China.
Department of Vascular Surgery, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
出版信息
Thromb Res. 2016 Jan;137:41-45. doi: 10.1016/j.thromres.2015.11.025. Epub 2015 Nov 26.
AIM
The purpose of this study was to explore the therapeutic efficacy of VEGF165-overexpressing vascular endothelial progenitor cells (EPCs) in post-thrombotic syndrome.
MATERIALS AND METHODS
A thrombus model was developed to mimic the in-vivo setting, and adenovirus transfection was used to overexpress VEGF165 in EPCs. These cells were transplanted into the animal model, and their ability to relieve endothelial injury was evaluated using haematoxylin and eosin staining, immunohistochemistry and scanning electron microscopy.
RESULTS
Ferric chloride was used to build rat models of the inferior vena cava thrombosis, and HEK 293A cells were used to amplify adenovirus that overexpresses VEGF165. EPCs were infected with adenovirus, and this was confirmed by fluorescence microscopy. Transplantation of VEGH165-overexpressing EPCs into injured endothelial sites led to faster repair of the post-thrombotic tunica intima than wild-type EPCs.
CONCLUSION
Transplantation of VEGF165-overexpressing EPCs was found to promote repair of the tunica intima, thus improving rehabilitation after surgery.
目的
本研究旨在探讨过表达血管内皮生长因子165（VEGF165）的血管内皮祖细胞（EPCs）在血栓形成后综合征中的治疗效果。
材料与方法
建立血栓模型以模拟体内情况，采用腺病毒转染使EPCs过表达VEGF165。将这些细胞移植到动物模型中，通过苏木精-伊红染色、免疫组织化学和扫描电子显微镜评估其减轻内皮损伤的能力。
结果
用氯化铁建立大鼠下腔静脉血栓形成模型，用HEK 293A细胞扩增过表达VEGF165的腺病毒。腺病毒感染EPCs，并通过荧光显微镜证实。将过表达VEGH165的EPCs移植到受损内皮部位，与野生型EPCs相比，血栓形成后内膜修复更快。
结论
发现过表达VEGF165的EPCs移植可促进内膜修复，从而改善术后康复。
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