Yan D, Dou Q L, Wang Z, Wei Y Y
Department of Pharmacology, Basic Medical College, Xinjiang Medical University, Xinjiang, China.
ICU, The First Affiliated Hospital of Xinjiang Medical University, Xinjiang, China.
Genet Mol Res. 2015 Nov 26;14(4):15224-32. doi: 10.4238/2015.November.25.10.
The objective of this study was to explore the experimental conditions for hepatocellular steatosis models of Chang liver cells induced by oleic acid (OA). For that, Chang liver cells were induced by different concentrations of OA for different periods. The MTT assay was used to detect hepatic cell activity, the Oil Red O staining was used to observe intracellular lipid droplets accumulation, and the glycerol phosphate oxidase method was used to detect the triglyceride (TG) content in the Chang liver cell. The hepatocellular steatosis models of Chang liver cell were established successfully by inducing with 0.2 mM OA for 24h. TG content in model cells was 379.98 ± 23.19 mg/g, which is significantly different from control cells (185.03 ± 12.68 mg/g; P < 0.01). These were considered proper conditions for establishing hepatocellular steatosis models of Chang liver cells, producing a reliable model for nonalcoholic fatty liver disease research.
本研究的目的是探索油酸（OA）诱导Chang肝细胞发生肝细胞脂肪变性模型的实验条件。为此，用不同浓度的OA对Chang肝细胞诱导不同时间。采用MTT法检测肝细胞活性，用油红O染色观察细胞内脂滴积累情况，并用磷酸甘油氧化酶法检测Chang肝细胞内甘油三酯（TG）含量。用0.2 mM OA诱导24小时成功建立了Chang肝细胞的肝细胞脂肪变性模型。模型细胞中TG含量为379.98±23.19 mg/g，与对照细胞（185.03±12.68 mg/g；P<0.01）有显著差异。这些被认为是建立Chang肝细胞脂肪变性模型的合适条件，为非酒精性脂肪性肝病研究提供了可靠的模型。
