Etzion Z, Perkins M E
Laboratory of Biochemical Parasitology, Rockefeller University, New York, NY 10021-6399.
Eur J Cell Biol. 1989 Apr;48(2):174-9.
Intracellular development of the malarial parasite results in substantial modifications of the membrane and cytoskeleton of the erythrocyte host cell. Two related Plasmodium falciparum-encoded proteins of 50 kDa and 43 kDa (Pf 50/43), identified by reactivity with a single mAb, were demonstrated to be localized to the erythrocyte cytoplasm of parasite-infected cells. Immunofluorescence and immunoelectron microscopy using mAb.7E11 demonstrated the Pf 50/43 is localized in the membrane of the vesicles in the erythrocyte cytoplasm, vesicles which correspond to Maurer's clefts. Solubility properties of the proteins suggest they are integral membrane proteins. By immunofluorescence, Pf 50/43 is shown to colocalize with actin which has a highly modified organization in the infected erythrocyte. Pf 50/43 is located exclusively in the vesicles, is not transported to the erythrocyte membrane or secreted. It is proposed the vesicles may play a role in transport of molecules across the erythrocyte cytoplasm, between the parasite and the external erythrocyte plasma membrane.
疟原虫的细胞内发育导致红细胞宿主细胞膜和细胞骨架发生显著改变。通过与一种单克隆抗体反应鉴定出两种相关的恶性疟原虫编码蛋白，分子量分别为50 kDa和43 kDa（Pf 50/43），它们定位于受寄生虫感染细胞的红细胞胞质中。使用单克隆抗体7E11进行免疫荧光和免疫电子显微镜观察表明，Pf 50/43定位于红细胞胞质中的囊泡膜上，这些囊泡与毛雷尔氏小体相对应。蛋白质的溶解性表明它们是整合膜蛋白。通过免疫荧光观察，Pf 50/43与肌动蛋白共定位，而肌动蛋白在受感染的红细胞中具有高度改变的组织结构。Pf 50/43仅位于囊泡中，不会转运至红细胞膜或分泌出去。有人提出，这些囊泡可能在分子跨红细胞胞质、在寄生虫与外部红细胞质膜之间的运输中发挥作用。
