Hiraoka A, Ohkubo T, Fukuda M
Department of Internal Medicine, Osaka Dental University, Japan.
Exp Cell Biol. 1989;57(1):27-34. doi: 10.1159/000163504.
Human hematopoietic survival and stem cell growth factor (SCGF), derived from the KPB-M15 myeloid cells, is a heat- and pH-stable protein. Chemical modification with various denaturing agents and proteolytic enzymes abrogated SCGF activity. The granulocyte-macrophage colony-potentiating and erythroid burst-promoting activities of SCGF were proportional to the density of the bone marrow (BM) cells cultured, the optimal BM cell density for delta granulocyte- and delta burst-promoting activities being 5 to 10 X 10(5)/ml. These data could be important in enabling the use of SCGF to induce proliferation of human hematopoietic stem cells in vitro.
源自KPB - M15髓样细胞的人造血存活和干细胞生长因子（SCGF）是一种热稳定和pH稳定的蛋白质。用各种变性剂和蛋白水解酶进行化学修饰可消除SCGF活性。SCGF的粒细胞 - 巨噬细胞集落增强活性和促红细胞爆式集落形成活性与培养的骨髓（BM）细胞密度成正比，δ粒细胞集落增强活性和δ爆式集落促进活性的最佳BM细胞密度为5至10×10⁵/ml。这些数据对于利用SCGF在体外诱导人造血干细胞增殖可能具有重要意义。
