Baral Samrat, Cho Du-Hyong, Pariyar Ramesh, Yoon Chi-Su, Chang Bo-Yoon, Kim Dae-Sung, Cho Hyoung-Kwon, Kim Sung Yeon, Oh Hyuncheol, Kim Youn-Chul, Kim Jaehyo, Seo Jungwon
Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan 570-749, Republic of Korea ; Hanbang Body-Fluid Research Center, Wonkwang University, Iksan 570-749, Republic of Korea.
Department of Pharmacology, School of Medicine, Eulji University, Jung-gu, Daejeon 301-746, Republic of Korea.
Evid Based Complement Alternat Med. 2015;2015:925432. doi: 10.1155/2015/925432. Epub 2015 Nov 9.
Myrrh has been used since ancient times for the treatment of various diseases such as inflammatory diseases, gynecological diseases, and hemiplegia. In the present study, we investigated the effects of aqueous extracts of myrrh resin (AEM) on scopolamine-induced memory impairments in mice. AEM was estimated with (2E,5E)-6-hydroxy-2,6-dimethylhepta-2,4-dienal as a representative constituent by HPLC. The oral administration of AEM for 7 days significantly reversed scopolamine-induced reduction of spontaneous alternation in the Y-maze test. In the passive avoidance task, AEM also restored the decreased latency time of the retention trial by scopolamine treatment. In addition, Western blot analysis and Immunohistochemistry revealed that AEM reversed scopolamine-decreased phosphorylation of Akt and extracellular signal-regulated kinase (ERK). Our study demonstrates for the first time that AEM ameliorates the scopolamine-induced memory impairments in mice and increases the phosphorylation of Akt and ERK in the hippocampus of mice brain. These results suggest that AEM has the therapeutic potential in memory impairments.
没药自古以来就被用于治疗各种疾病，如炎症性疾病、妇科疾病和偏瘫。在本研究中，我们研究了没药树脂水提取物（AEM）对东莨菪碱诱导的小鼠记忆障碍的影响。通过高效液相色谱法以（2E，5E）-6-羟基-2，6-二甲基庚-2，4-二烯醛作为代表性成分对AEM进行了评估。连续7天口服AEM可显著逆转东莨菪碱诱导的Y迷宫试验中自发交替行为的减少。在被动回避任务中，AEM还恢复了经东莨菪碱处理后记忆保持试验中缩短的潜伏期。此外，蛋白质免疫印迹分析和免疫组织化学显示，AEM可逆转东莨菪碱降低的Akt和细胞外信号调节激酶（ERK）的磷酸化水平。我们的研究首次证明，AEM可改善东莨菪碱诱导的小鼠记忆障碍，并增加小鼠脑海马体中Akt和ERK的磷酸化水平。这些结果表明，AEM在记忆障碍方面具有治疗潜力。
