Siengdee Puntita, Radeerom Tiwaporn, Kuanoon Similan, Euppayo Thippaporn, Pradit Waranee, Chomdej Siriwadee, Ongchai Siriwan, Nganvongpanit Korakot
Animal Bone and Joint Research Laboratory, Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand.
BMC Vet Res. 2015 Dec 4;11:298. doi: 10.1186/s12917-015-0611-6.
Intra-articular injection of corticosteroids is used to treat the inflammatory pain of arthritis and osteoarthritis (OA), but our previous study found a deleterious effect of these steroids on chondrocyte cells. Hyaluronic acid (HA) injection has been suggested as a means to counteract negative side effects through replenishment of synovial fluid that can decrease pain in affected joints. To better understand the effects of corticosteroids on these processes, dexamethasone (Dex) and prednisolone (Pred) were administered to porcine cartilage explants at several concentrations with and without HA. We examined corticoid effects by determining sulfate-glycosaminoglycan (s-GAG) and uronic acid (UA) content of the explant media, and safranin-O staining of the cells. Analysis of lactate dehydrogenase (LDH) activity was conducted to assess cell cytotoxicity.
Dex treatment significantly reduced cellular cytotoxicity compared to the other treatment groups, especially with regards to the release of s-GAG, and protects against superficial proteoglycan damage. However, there was no difference between Pred and Dex, with and without HA, in the UA content remaining in porcine cartilage explants.
The data suggest that combinations of Dex and Pred with HA did not have a significant effect on protection or enhancement of the articular cartilage matrix under the current conditions.
关节腔内注射皮质类固醇用于治疗关节炎和骨关节炎（OA）的炎性疼痛，但我们之前的研究发现这些类固醇对软骨细胞有有害影响。有人提出注射透明质酸（HA）作为一种手段，通过补充滑液来抵消副作用，滑液可减轻患关节的疼痛。为了更好地了解皮质类固醇对这些过程的影响，将地塞米松（Dex）和泼尼松龙（Pred）以几种浓度分别给予猪软骨外植体，有或没有HA。我们通过测定外植体培养基中的硫酸糖胺聚糖（s-GAG）和糖醛酸（UA）含量以及细胞的番红O染色来检查皮质激素的作用。进行乳酸脱氢酶（LDH）活性分析以评估细胞毒性。
与其他治疗组相比，Dex治疗显著降低了细胞毒性，特别是在s-GAG释放方面，并防止了表面蛋白聚糖损伤。然而，在有或没有HA的情况下，猪软骨外植体中剩余的UA含量在Pred和Dex之间没有差异。
数据表明，在当前条件下，Dex和Pred与HA的组合对关节软骨基质的保护或增强没有显著影响。
