School of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.
School of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea; Department of Chemical Engineering, COMSATS Institute of Information Technology (CIIT), Lahore, Pakistan.
Bioresour Technol. 2016 Feb;201:74-9. doi: 10.1016/j.biortech.2015.11.030. Epub 2015 Nov 19.
This study determines and compares the intrinsic kinetic parameters (Ks and Ki) of selected Thermococcus onnurineus NA1 strains (wild-type (WT), and mutants MC01, MC02, and WTC156T) using the substrate inhibition model. Ks and Ki values were used to find the optimum dissolved CO (CL) conditions inside the reactor. The results showed that in terms of the maximum specific CO consumption rates (qCO(max)) of WT, MC01, MC02, and WTC156T the optimum activities can be achieved by maintaining the CL levels at 0.56mM, 0.52mM, 0.58mM, and 0.75mM, respectively. The qCO(max) value of WTC156T at 0.75mM was found to be 1.5-fold higher than for the WT strain, confirming its superiority. Kinetic modeling was then used to predict the conditions required to maintain the optimum CL levels and high cell concentrations in the reactor, based on the kinetic parameters of the WTC156T strain.
本研究采用底物抑制模型确定并比较了选定的 Thermococcus onnurineus NA1 菌株（野生型（WT）和突变株 MC01、MC02 和 WTC156T）的内在动力学参数（Ks 和 Ki）。Ks 和 Ki 值用于寻找反应器内最佳溶解 CO（CL）条件。结果表明，就 WT、MC01、MC02 和 WTC156T 的最大比 CO 消耗速率（qCO(max))而言，通过将 CL 水平分别维持在 0.56mM、0.52mM、0.58mM 和 0.75mM，可以达到最佳活性。在 0.75mM 时，WTC156T 的 qCO(max)值比 WT 菌株高 1.5 倍，证实了其优越性。然后，基于 WTC156T 菌株的动力学参数，使用动力学模型来预测在反应器中维持最佳 CL 水平和高细胞浓度所需的条件。
