Bae Seung Seob, Lee Hyun Sook, Jeon Jung Ho, Lee Jung-Hyun, Kang Sung Gyun, Kim Tae Wan
Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon, 305-333, Republic of Korea.
Bioprocess Biosyst Eng. 2015 May;38(5):989-93. doi: 10.1007/s00449-014-1336-9. Epub 2014 Dec 24.
Hyperthermophilic archaeon, Thermococcus onnurineus NA1 was reported to grow on formate producing hydrogen (H2). In this study, to sustain high H2 production rate and demonstrate the feasibility of mass production of H2, high cell density cultivation of T. onnurineus NA1 on sodium formate was employed under optimized conditions. From batch cultures, it was observed that the salinity of medium, significantly changed by the addition of formate salt and pH-adjusting agent, crucially affected cell growth and H2 production. With salinity carefully controlled between 3.7 and 4.6 %, 400 mM sodium formate was found to be an optimal initial concentration for maximizing cell growth-associated H2 production. Under optimal conditions, the repeated batch culture with cell recycling showed high cell density of OD600 of 1.7 in 3 and 30 L bioreactor, and the volumetric H2 production rate was enhanced up to 235.7 mmol L(-1) h(-1), which is one of the highest values reported to date.
据报道，嗜热古菌嗜热栖热放线菌NA1能够利用甲酸盐生长并产生氢气（H₂）。在本研究中，为了维持高氢气产生速率并证明大规模生产氢气的可行性，在优化条件下采用甲酸钠对嗜热栖热放线菌NA1进行高细胞密度培养。从分批培养中观察到，甲酸盐和pH调节剂的添加显著改变了培养基的盐度，这对细胞生长和氢气产生至关重要。当盐度小心控制在3.7%至4.6%之间时，发现400 mM甲酸钠是使与细胞生长相关的氢气产生最大化的最佳初始浓度。在最佳条件下，采用细胞循环的重复分批培养在3 L和30 L生物反应器中显示出OD600为1.7的高细胞密度，并且体积氢气产生速率提高到235.7 mmol L⁻¹ h⁻¹，这是迄今为止报道的最高值之一。
