Sontheimer R D, Matsubara T, Seelig L L
Department of Dermatology, University of Texas Southwestern Medical Center, Dallas 75235-9069.
J Invest Dermatol. 1989 Jul;93(1):154-9. doi: 10.1111/1523-1747.ep12277390.
A previously uncharacterized population of class II antigen-bearing dendritic cells that are intimately associated with the dermal microvasculature was identified in normal human skin using a double-label, indirect immunofluorescence technique. The only other major HLA-DR positive dermal cell type noted in these studies, the dermal microvascular endothelial cell (DMVEC), appeared to express lesser amounts of HLA-DR region gene product than did this dermal perivascular dendritic cell (DPDC). These DPDC were particularly common around small vessels in the superficial vascular plexus of the papillary dermis and were distinct from the mast cell, another cell type normally seen in a similar location. Phenotypic and ultrastructural studies have determined that the DPDC is more closely related to the monocyte/macrophage lineage than the dendritic cell lineage. The perivascular location and phenotype of this cell distinguishes it from other previously described constitutive dermal cell types such as the classic "histiocyte," veiled cell, and dendrocyte. The relatively rich expression of all three major HLA-D region gene products by this dermal perivascular dendritic macrophage would suggest that it could play a significant role in the immunobiology of the dermal microvascular unit.
利用双标记间接免疫荧光技术，在正常人类皮肤中鉴定出了一类与真皮微血管密切相关的、此前未被描述过的表达II类抗原的树突状细胞群体。在这些研究中发现的唯一其他主要的HLA - DR阳性真皮细胞类型，即真皮微血管内皮细胞（DMVEC），其表达的HLA - DR区域基因产物似乎比这种真皮血管周围树突状细胞（DPDC）少。这些DPDC在乳头真皮浅层血管丛的小血管周围特别常见，并且与肥大细胞不同，肥大细胞是通常在类似位置可见的另一种细胞类型。表型和超微结构研究已确定，DPDC与单核细胞/巨噬细胞谱系的关系比与树突状细胞谱系的关系更为密切。这种细胞的血管周围位置和表型使其与其他先前描述的固有真皮细胞类型，如经典的“组织细胞”、面纱细胞和树突状细胞有所区别。这种真皮血管周围树突状巨噬细胞对所有三种主要HLA - D区域基因产物的相对丰富表达表明，它可能在真皮微血管单位的免疫生物学中发挥重要作用。
