Liao Naishun, Huang Yunmei, Ye Jinxia, Chen Wenlie, Li Zuan Fang, Lin Ruhui, Li Xihai, Zheng Liangpu, Liu Xianxiang
Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.
Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China ; Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China ; National Laboratory of Traditional Chinese Medicine Pharmacology (Cell Structure and Function), Fujian Academy of Integrative Medicine, Fuzhou, Fujian 350122, P.R. China.
Exp Ther Med. 2015 Nov;10(5):1908-1914. doi: 10.3892/etm.2015.2739. Epub 2015 Sep 10.
Tumor necrosis factor-α (TNF-α) plays an important role in the abnormal metabolism of osteoblasts (OBs), which leads to subchondral bone (SB) alterations in osteoarthritis. In the present study, Tougu Xiaotong capsule (TXC), a traditional Chinese medicine, was used to treat TNF-α-injured OB-like cells. The cellular viability, mortality and ultramicroscopic morphology were evaluated. Thereafter, the activity of alkaline phosphatase (ALP), secretion of osteocalcin (OCN) and mineralization of nodules were analyzed. The results showed that TXC treatment significantly promoted cell proliferation, reduced cellular mortality and improved cellular ultrastructure, particularly that of the endoplasmic reticulum and nucleus. These data indicate that TXC is able to promote cell growth, as well as prevent inflammation in OB-like cells. Furthermore, the activity of ALP, secretion of OCN and mineralization of nodules were accelerated, and the calcium content of the TNF-α-injured OB-like cells was promoted by TXC treatment. These results indicate that TXC protected the OB-like cells from TNF-α-induced injuries. This may be a potential mechanism through which TXC regulates SB remodeling in the clinical treatment of osteoarthritis.
肿瘤坏死因子-α（TNF-α）在成骨细胞（OBs）的异常代谢中起重要作用，这会导致骨关节炎中软骨下骨（SB）的改变。在本研究中，中药透骨消痛胶囊（TXC）被用于治疗TNF-α损伤的类成骨细胞。评估了细胞活力、死亡率和超微形态。此后，分析了碱性磷酸酶（ALP）的活性、骨钙素（OCN）的分泌和结节的矿化情况。结果表明，TXC处理显著促进细胞增殖，降低细胞死亡率并改善细胞超微结构，尤其是内质网和细胞核的超微结构。这些数据表明，TXC能够促进细胞生长，并预防类成骨细胞中的炎症。此外，TXC处理加速了ALP的活性、OCN的分泌和结节的矿化，并且促进了TNF-α损伤的类成骨细胞的钙含量。这些结果表明，TXC保护类成骨细胞免受TNF-α诱导的损伤。这可能是TXC在骨关节炎临床治疗中调节SB重塑的潜在机制。
