Dawson Deborah A, Kleven Oddmund, Dos Remedios Natalie, Horsburgh Gavin J, Kroglund Rolf T, Santos Teresa, Hewitt Colin R A
Department of Animal and Plant Sciences, University of Sheffield, Western Bank, Sheffield, South Yorkshire, S10 2TN UK.
Norwegian Institute for Nature Research (NINA), 7485 Trondheim, Norway.
Conserv Genet Resour. 2015;7(4):887-894. doi: 10.1007/s12686-015-0497-4. Epub 2015 Oct 29.
During the 1950s and 1970s the osprey () experienced a dramatic population crash and remains of conservation concern in several parts of the world. We isolated 37 microsatellite loci and assessed these in ospreys sampled in the UK and Norway (using mouth swabs/feathers). From 26 loci variable in four ospreys, we selected 13, combined these into two multiplex-PCR sets and included a sex-typing marker. Additional markers confirmed sexes. In 17 ospreys, feather-sampled in central Norway, we found 3-10 alleles per locus. The 13 loci are autosomal (heterozygotes were present in both sexes) and observed heterozygosities ranged from 0.24 to 0.94. The combined probability of identity for the 13 loci was 8.0 × 10. These microsatellite loci will be useful for genetic monitoring, parentage analysis and population genetic studies of the osprey.
在20世纪50年代至70年代期间，鹗（）的种群数量急剧下降，在世界上的几个地区仍然受到保护关注。我们分离出37个微卫星位点，并在英国和挪威采集的鹗样本（使用口腔拭子/羽毛）中对这些位点进行了评估。从在四只鹗中可变的26个位点中，我们选择了13个，将它们组合成两个多重PCR组，并包括一个性别分型标记。其他标记证实了性别。在挪威中部采集羽毛样本的17只鹗中，我们发现每个位点有3至10个等位基因。这13个位点是常染色体的（两性中均存在杂合子），观察到的杂合度范围为0.24至0.94。这13个位点的联合个体识别概率为8.0×10。这些微卫星位点将有助于鹗的遗传监测、亲权分析和种群遗传学研究。
