Al-Hosary Amira A T, Ahmed Jabbar, Nordengrahn Ann, Merza Malik
Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt.
Institute of Parasitology and Tropical Veterinary Medicine, Faculty of Veterinary Medicine, Free University of Berlin, 14195 Berlin, Germany.
J Parasitol Res. 2015;2015:787812. doi: 10.1155/2015/787812. Epub 2015 Nov 10.
The present study assesses the efficacy of SVANOVIR Theileria annulata-Ab, the first commercial ELISA kit for the diagnosis of Theileria annulata infection in cattle based on a recombinant protein known as T. annulata surface protein (TaSp). As a reference test, a polymerase chain reaction (PCR) assay depending on T. annulata merozoite surface antigen (Tams-1) was applied. A total of 468 blood samples as well as serum samples were randomly collected from cattle and tested in the PCR as well as in the ELISA developed in this study. Moreover, all samples were also analyzed by conventional Giemsa-stained blood smear. The results of this study revealed a good correlation between the results obtained by PCR and the ELISA, whereas all PCR positive samples scored correctly positive in the ELISA and 73 of the 125 PCR negative samples scored correctly negative. Taken together, a sensitivity of 91.25% and a specificity of 78.4% were recorded, when compared to the PCR data. In conclusion, the SVANOVIR Theileria annulata-Ab is a suitable diagnostic assay for use in the diagnosis and epidemiological surveys of Theileria annulata infection in chronic and carrier animals.
本研究评估了SVANOVIR环形泰勒虫抗体（SVANOVIR Theileria annulata-Ab）的效力，这是首个基于一种名为环形泰勒虫表面蛋白（TaSp）的重组蛋白用于诊断牛环形泰勒虫感染的商业酶联免疫吸附测定（ELISA）试剂盒。作为参考检测方法，采用了一种基于环形泰勒虫裂殖子表面抗原（Tams-1） 的聚合酶链反应（PCR）检测法。从牛身上随机采集了总共468份血液样本和血清样本，并在本研究开发的PCR检测法和ELISA检测法中进行检测。此外，所有样本还通过传统的吉姆萨染色血涂片进行分析。本研究结果显示，PCR检测法和ELISA检测法所得结果之间具有良好的相关性，所有PCR阳性样本在ELISA检测中均正确判定为阳性，125份PCR阴性样本中有73份在ELISA检测中正确判定为阴性。与PCR数据相比，总体灵敏度为91.25%，特异性为78.4%。总之，SVANOVIR环形泰勒虫抗体是一种适用于慢性感染动物和带菌动物环形泰勒虫感染诊断及流行病学调查的诊断检测方法。
