Ni Qian, Wan Feng Qi, Jing Yu Hong, Dong Xiang Yu, Zhang You Cheng
Department of Pediatrics, Lanzhou University Second Hospital, Lanzhou 730000, China.
Laboratory of Pediatrics, Lanzhou University Second Hospital, Lanzhou 730000, China.
Anal Cell Pathol (Amst). 2015;2015:159549. doi: 10.1155/2015/159549. Epub 2015 Nov 12.
In this study, we aimed to investigate the effect of acute and chronic exposure to HA on the aerobic and anaerobic metabolism in liver by determining the hepatic levels of ICDH and ATP. Lactate levels in liver and blood were also examined. Rats were exposed to an altitude of 4,300 m for 30 days, and those without HA exposure were used as controls. We observed an increased expression of liver ICDH following acute exposure (days 1, 3, and 7), whereas the liver ATP concentration was reduced on day 1. No changes in the hepatic expression of ICDH and ATP were found in rats chronically exposed to HA. Lactate concentrations of liver and blood did not show any significant changes following HA exposure. Thus, aerobic metabolism may be the major metabolic pathway in response to HA hypoxia in order to acclimatize themselves to the stressful environments.
在本研究中，我们旨在通过测定肝脏中异柠檬酸脱氢酶（ICDH）和三磷酸腺苷（ATP）的水平，研究急性和慢性暴露于高原低氧环境（HA）对肝脏有氧和无氧代谢的影响。同时也检测了肝脏和血液中的乳酸水平。将大鼠暴露于4300米海拔高度30天，未暴露于高原低氧环境的大鼠作为对照。我们观察到急性暴露（第1、3和7天）后肝脏ICDH表达增加，而第1天肝脏ATP浓度降低。长期暴露于高原低氧环境的大鼠肝脏中ICDH和ATP的表达没有变化。高原低氧环境暴露后，肝脏和血液中的乳酸浓度没有显著变化。因此，有氧代谢可能是机体应对高原低氧环境缺氧的主要代谢途径，以便使自身适应应激环境。
