Li Bo, Yao Yongfang, Li Diyan, Ni Qingyong, Zhang Mingwang, Xie Meng, Xu Huailiang
a College of Life Science, Sichuan Agricultural University , Ya'an , China , and.
b College of Animal Science and Technology, Sichuan Agricultural University , Chengdu , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2017 Jan;28(1):21-22. doi: 10.3109/19401736.2015.1106524. Epub 2015 Dec 7.
We sequenced the complete mitochondrial DNA genome of Yuhina diademata by means of polymerase chain reaction. The circle genome (17 834 bp in length) contained 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes, and 2 control regions (Dloop1 and Dloop2), which was similar to the other published mitogenome of Timaliidae species. Most PCGs began with ATG, while COI and ND2 genes started with GTG. Nine of the PCGs used complete (TAA) or incomplete (TA- or T--) stop codon, while ND1 and ND6 genes ended with AGA, COI gene ended with AGG, and ND6 gene with TAG. Overall base composition of the complete mtDNA was 30.30% A, 23.81% T, 31.72% C, and 14.17% G. All the PCGs in Y. diademata were distributed on the H-strand, except for the ND6 gene and eight tRNA genes which were encoded on the L-strand.
我们通过聚合酶链反应对冕羽画眉的完整线粒体DNA基因组进行了测序。环状基因组(长度为17834 bp)包含13个蛋白质编码基因(PCGs)、2个rRNA基因、22个tRNA基因和2个控制区(Dloop1和Dloop2),这与其他已发表的画眉科物种的线粒体基因组相似。大多数PCGs以ATG起始,而COI和ND2基因以GTG起始。9个PCGs使用完整的(TAA)或不完整的(TA-或T--)终止密码子,而ND1和ND6基因以AGA结尾,COI基因以AGG结尾,ND6基因以TAG结尾。完整线粒体DNA的总体碱基组成为30.30%A、23.81%T、31.72%C和14.17%G。冕羽画眉的所有PCGs分布在H链上,除了ND6基因和8个tRNA基因编码在L链上。