Chang Zheng, Shen Qing-Qing
a College of Environment and Resources, Wenshan University , Wenshan , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4561-4562. doi: 10.3109/19401736.2015.1101564. Epub 2015 Nov 5.
The complete mitochondrial genome of the navel orangeworm Amyelois transitella (Insecta: Lepidoptera: Pyralidae) was assembled from Illumina sequencing reads (Illumina Inc., San Diego, CA). The genome is a double-stranded circular molecule of 15 205 bp, comprising 22 transfer RNAs (tRNAs), 13 protein-coding genes (PCGs), two ribosomal RNAs (rRNAs), and one control region. All PCGs are initiated with ATN codons, except for COX1 with the putative CGA initiation codon. Six PCGs (COX1, COX2, CYTB, ND2, ND3, and ND4) harbor an incomplete termination codon T, while all the others are terminated with TAA (ATP6, ATP8, COX3, ND1, ND4, and ND6) or TAG (ND4L). The base composition is highly biased (37.9% A, 12.6% C, 7.8% G, and 41.7% T) with an overall A + T content of 79.6%. Phylogenetic analysis indicated that A. transitella is more phylogenetically related to its confamilial counterparts than to those from the family Crabidae.
利用Illumina测序读数(Illumina公司,加利福尼亚州圣地亚哥)组装了脐橙螟Amyelois transitella(昆虫纲:鳞翅目:螟蛾科)的完整线粒体基因组。该基因组是一个15205bp的双链环状分子,由22个转移RNA(tRNA)、13个蛋白质编码基因(PCG)、两个核糖体RNA(rRNA)和一个控制区组成。除COX1的起始密码子推测为CGA外,所有PCG均以ATN密码子起始。6个PCG(COX1、COX2、CYTB、ND2、ND3和ND4)含有不完整的终止密码子T,而其他所有PCG均以TAA(ATP6、ATP8、COX3、ND1、ND4和ND6)或TAG(ND4L)终止。碱基组成高度偏向(A占37.9%、C占12.6%、G占7.8%、T占41.7%),A+T总含量为79.6%。系统发育分析表明,脐橙螟与其同科对应物种的亲缘关系比与蟹科物种的亲缘关系更近。