Safaei Zahra, Alipour Eskandar, Shafaati Alireza, Zarghp Afshin
Acta Pol Pharm. 2015 Mar-Apr;72(2):227-33.
A rapid, simple and reproducible high performance liquid chromatographic method was developed and validated for determination of fluconazole in human plasma. The separation was performed on MZ C8 column (125 x 4 mm, 5 µm) using acetonitrile - potassium dihydrogen phosphate buffer (15 : 85, v/v), pH 3.0, as the mobile phase at a flow rate of 1.5 mL/min. The wavelength was set at 261 nm. The assay enables the measurement of fluconazole for therapeutic drug monitoring with a minimum quantification limit of 20 ng/mL. The method involves simple, protein precipitation procedure and analytical recovery was complete. The calibration curve was linear over the concentration range 0.1-4 µg/mL. The coefficients of variation for inter-day and intra-day assay were found to be less than 10%.
建立并验证了一种快速、简单且可重复的高效液相色谱法，用于测定人血浆中的氟康唑。采用MZ C8柱（125×4 mm，5 µm）进行分离，以乙腈-磷酸二氢钾缓冲液（15:85，v/v），pH 3.0作为流动相，流速为1.5 mL/min。检测波长设定为261 nm。该测定法可用于氟康唑的治疗药物监测，最低定量限为20 ng/mL。该方法采用简单的蛋白沉淀程序，分析回收率完全。校准曲线在0.1-4 µg/mL的浓度范围内呈线性。日间和日内测定的变异系数均小于10%。
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