Departamento de Farmacia, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, #6 Ave. Manuel Nava, San Luis Potosí, C.P.78210, México.
Hospital Central "Dr. Ignacio Morones Prieto", #2395 Ave. Venustiano Carranza, San Luis Potosí, C.P.78290, México.
Bioanalysis. 2024;16(19-20):1045-1053. doi: 10.1080/17576180.2024.2387452. Epub 2024 Sep 5.
An accurate and fast ultra-high performance liquid chromatography coupled with tandem mass spectrometry analytical method was developed and validated for quantifying fluconazole levels in human plasma according to the US FDA guidelines. A simple protein precipitation by acetonitrile was employed for the sample preparation. The chromatographic separation was carried out using isocratic elution of water (0.1% formic acid) and acetonitrile on an Acquity ultra-high performance liquid chromatography HSS T3 column. Samples from ten adult patients diagnosed with candidemia who received fluconazole treatment were analyzed. The method demonstrated excellent linearity and stability within the 1-50 μg/ml range (r >0.999). The intraday and interday precision were determined with coefficient of variation values ranging from 1.4 to 4.38% and 2.8 to 6.6%, respectively. This rapid and selective method has successfully analyzed 27 plasma samples. The straightforward sample preparation in a single step and the reduced analysis time make this method suitable for adult patients with candidemia, leading to improved clinical outcomes.
建立并验证了一种准确、快速的超高效液相色谱-串联质谱分析方法，用于根据美国 FDA 指南定量检测人血浆中的氟康唑浓度。采用乙腈简单沉淀蛋白进行样品制备。采用 Acquity UPLC HSS T3 柱，以水（0.1%甲酸）和乙腈的等度洗脱进行色谱分离。分析了 10 名接受氟康唑治疗的确诊为念珠菌血症的成年患者的样本。该方法在 1-50μg/ml 范围内表现出优异的线性和稳定性（r>0.999）。日内和日间精密度的变异系数分别为 1.4-4.38%和 2.8-6.6%。该快速、选择性方法已成功分析了 27 个血浆样本。该方法仅需一步即可进行简单的样品制备，且分析时间较短，非常适合念珠菌血症的成年患者，从而改善了临床结局。
