Huang Jian-Qing, Liang Hong-Ling, Jin Tian-En, Xie Zhi
Cancer Center of Guangzhou Medical University Guangzhou 510095, China.
Guangdong Lung Cancer Institute, Guangdong General Hospital Guangzhou 510080, China.
Int J Clin Exp Pathol. 2015 Sep 1;8(9):11278-86. eCollection 2015.
Apoptosis-related molecules can be abnormally expressed in cancers and underscore the hallmark of resisting cell death in cancer cells. This study was aimed to observe the expression patterns of apoptosis-related molecules in lung cancer and paired non-cancerous tissues, and to observe if there is a correlation between the expression of these apoptotic molecules and clinicopathologic parameters. Immunohistochemistry (IHC) was performed to analyze the expression level of CASP3, CASP8, CASP9, PARP1, Cleaved CASP3 (C-CASP3), Cleaved PARP1 (C-PARP1), XIAP, BIRC5 (Survivin) and BCL2 in lung cancer and paired non-cancerous tissues. We found that apoptosis-related molecules CASP3, CASP9, BCL2, BIRC5 and PARP1 are abnormally expressed in lung cancer cells and their expression were correlated with histology. BCL2, BIRC5 and PARP1 are expressed at higher levels in SCC than in non-SCC. C-PARP1 expression might be an independent prognostic factor for NSCLC.
凋亡相关分子在癌症中可异常表达，并突显了癌细胞抵抗细胞死亡的特征。本研究旨在观察凋亡相关分子在肺癌及配对的癌旁组织中的表达模式，并观察这些凋亡分子的表达与临床病理参数之间是否存在相关性。采用免疫组织化学（IHC）方法分析肺癌及配对癌旁组织中CASP3、CASP8、CASP9、PARP1、裂解的CASP3（C-CASP3）、裂解的PARP1（C-PARP1）、XIAP、BIRC5（存活素）和BCL2的表达水平。我们发现凋亡相关分子CASP3、CASP9、BCL2、BIRC5和PARP1在肺癌细胞中异常表达，且它们的表达与组织学相关。BCL2、BIRC5和PARP1在鳞状细胞癌（SCC）中的表达水平高于非SCC。C-PARP1的表达可能是非小细胞肺癌（NSCLC）的一个独立预后因素。
