Begum Anjuman Ara, Jakaria D M, Anisuzzaman Sharif Md, Islam Mahfuzul, Mahmud Siraje Arif
Department of Pharmacy, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh.
Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh.
J Pharm (Cairo). 2015;2015:763796. doi: 10.1155/2015/763796. Epub 2015 Nov 16.
Probiotics containing food supplements available in Bangladesh market were identified and collected for assessment. To assess their label claim, they were resuspended into sterile distilled water. Then, series dilutions of each sample solution were prepared and immediately plated out, in duplicate, into De Man Rogosa Sharpe (MRS) agar. These plates were then incubated at 37°C for 48 hours and colonies were counted. Viable cell numbers stated on the labels were compared with actual viable cell numbers. To assess the viability of the probiotics included in the products, probiotic strains were isolated from each of the four products and screened for inhibitory activity against six indicator strains. It was surprisingly found that although the viable cell numbers of all supplements were three to four log cycles lower than label claim of the products, however, this problem did not affect the inhibitory activity of the probiotic strains against indicator strains according to in vitro assessment. Legislation and regulation regarding prebiotic-probiotic containing products should be built up in Bangladesh to ensure quality products supply to the consumers. Moreover, manufacturers of probiotic containing products should take the responsibility for providing the consumer with scientifically and legally correct information.
识别并收集了孟加拉国市场上含益生菌的食品补充剂,用于评估。为评估其标签声明,将它们重悬于无菌蒸馏水中。然后,制备每个样品溶液的系列稀释液,并立即一式两份接种到德氏乳杆菌-罗格斯-夏普(MRS)琼脂平板上。接着将这些平板在37°C下孵育48小时并计数菌落。将标签上标明的活细胞数与实际活细胞数进行比较。为评估产品中所含益生菌的活力,从四种产品中分别分离出益生菌菌株,并筛选其对六种指示菌株的抑制活性。令人惊讶的是,虽然所有补充剂的活细胞数比产品的标签声明低三到四个对数周期,然而,根据体外评估,这个问题并未影响益生菌菌株对指示菌株的抑制活性。孟加拉国应建立关于含益生元-益生菌产品的法律法规,以确保向消费者提供优质产品。此外,含益生菌产品的制造商应负责向消费者提供科学和合法的正确信息。