Wang Xintao, Wang Pijun, Wang Wenjun, Murray John W, Wolkoff Allan W
Marion Bessin Liver Research Center, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY, 10461, USA.
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY, 10461, USA.
Traffic. 2016 Mar;17(3):230-44. doi: 10.1111/tra.12354. Epub 2016 Jan 10.
Na(+)-taurocholate cotransporting polypeptide (ntcp) mediates bile acid transport, also serving as the hepatitis B virus receptor. It traffics in vesicles along microtubules, requiring activity of protein kinase C (PKC)ζ for motility. We have now found that the epidermal growth factor receptor (EGFR) is the target of PKCζ activity and that EGFR and ntcp colocalize in vesicles. ntcp-containing vesicles that are not associated with EGFR have reduced microtubule-based motility, consistent with intracellular accumulation and reduced surface expression of ntcp in cells following EGFR knockdown.
牛磺胆酸钠共转运多肽（NTCP）介导胆汁酸转运，同时也是乙肝病毒受体。它沿微管在囊泡中运输，其运动需要蛋白激酶C（PKC）ζ的活性。我们现在发现表皮生长因子受体（EGFR）是PKCζ活性的靶点，且EGFR与NTCP在囊泡中共定位。不与EGFR相关的含NTCP囊泡基于微管的运动减少，这与EGFR敲低后细胞内NTCP积累及表面表达降低相一致。
