Araujo Pedro, Tilahun Ephrem, Breivik Joar Fjørtoft, Abdulkader Bashir M, Frøyland Livar, Zeng Yingxu
National Institute of Nutrition and Seafood Research (NIFES), PO Box 2029 Nordnes, N-5817 Bergen, Norway.
National Institute of Nutrition and Seafood Research (NIFES), PO Box 2029 Nordnes, N-5817 Bergen, Norway.
Talanta. 2016 Feb 1;148:463-71. doi: 10.1016/j.talanta.2015.11.016. Epub 2015 Nov 11.
It is well-known that triacylglycerol (TAG) ions are suppressed by phospholipid (PL) ions in regiospecific analysis of TAG by mass spectrometry (MS). Hence, it is essential to remove the PL during sample preparation prior to MS analysis. The present article proposes a cost-effective liquid-liquid extraction (LLE) method to remove PL from TAG in different kinds of biological samples by using methanol, hexane and water. High performance thin layer chromatography confirmed the lack of PL in krill oil and salmon liver samples, submitted to the proposed LLE protocol, and liquid chromatography tandem MS confirmed that the identified TAG ions were highly enhanced after implementing the LLE procedure.
众所周知，在通过质谱法（MS）对三酰甘油（TAG）进行区域特异性分析时，磷脂（PL）离子会抑制TAG离子。因此，在进行MS分析之前的样品制备过程中去除PL至关重要。本文提出了一种经济高效的液液萃取（LLE）方法，通过使用甲醇、己烷和水从不同种类的生物样品中的TAG中去除PL。高效薄层色谱法证实，按照所提出的LLE方案处理后的磷虾油和鲑鱼肝样品中不存在PL，液相色谱串联质谱法证实，实施LLE程序后，所鉴定的TAG离子得到了高度增强。
