Xu Kai Wei, Zou Lan, Penttinen Petri, Zeng Xiangzhong, Liu Ming, Zhao Ke, Chen Cuiping, Chen Yuan Xue, Zhang Xiaoping
Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu 625014, China.
Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu 625014, China.
Syst Appl Microbiol. 2016 Feb;39(1):33-40. doi: 10.1016/j.syapm.2015.10.005. Epub 2015 Dec 1.
Thirty-four rhizobial isolates were obtained from root nodules of four wild Desmodium species growing in Panxi, Sichuan, China. According to the combined ARDRA and IGS-RFLP (CACAI) cluster analysis, Rhizobium, Pararhizobium and Mesorhizobium isolates outnumbered Bradyrhizobium isolates. In general, the isolates representing the same species from the same site clustered together. Furthermore, the four Desmodium species were all nodulated by more than one rhizobial species. AFLP and phenotypic analyses showed that the 34 isolates represented at least 32 distinct strains. None of the strains were found from more than one site or host, indicating a high degree of rhizobial diversity in Panxi. In the multilocus sequence analysis, the isolates were assigned to Pararhizobium giardinii, Bradyrhizobium japonicum, Mesorhizobium septentrionale, and to undescribed species of the genera Rhizobium, Bradyrhizobium and Agrobacterium.
从生长在中国四川攀西地区的4种野生山蚂蝗属植物的根瘤中获得了34株根瘤菌分离物。根据ARDRA和IGS-RFLP（CACAI）联合聚类分析，根瘤菌属、副根瘤菌属和中慢生根瘤菌属的分离物数量超过慢生根瘤菌属的分离物。一般来说，来自同一地点的代表同一物种的分离物聚集在一起。此外，这4种山蚂蝗属植物均被不止一种根瘤菌物种结瘤。AFLP和表型分析表明，这34株分离物代表至少32个不同的菌株。没有一个菌株是从不止一个地点或宿主中发现的，这表明攀西地区根瘤菌具有高度的多样性。在多位点序列分析中，这些分离物被归类为贾氏副根瘤菌、日本慢生根瘤菌、北方中慢生根瘤菌，以及根瘤菌属、慢生根瘤菌属和土壤杆菌属中未描述的物种。
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