Kim Kwang-Youl, Cho Sang-Heon, Song Yong-Hyun, Nam Moon-Suk, Kim Cheol-Woo
Department of Clinical Pharmacology, Inha University Hospital, Inha University School of Medicine, Incheon, South Korea.
Department of Clinical Pharmacology, Inha University Hospital, Inha University School of Medicine, Incheon, South Korea; Department of Internal Medicine, Inha University Hospital, Inha University School of Medicine, Incheon, South Korea.
J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Jan 1;1008:125-131. doi: 10.1016/j.jchromb.2015.11.037. Epub 2015 Nov 24.
A direct injection-based, simple, accurate, and robust LC-MS/MS method was developed and validated for the determination of teicoplanin in human plasma. Patient plasma samples were diluted in an aqueous buffer prior to injection into the LC-MS/MS system. Chromatographic separation was achieved using a Cadenza HS-C18 column and a gradient mixture of acetonitrile-water (both containing 0.1% formic acid) as the mobile phase at a flow rate of 0.5mL/min. The analytes were detected in multiple reaction monitoring mode with positive ion electrospray ionization. The concentration of teicoplanin was determined as the sum of six components (A3-1, A2-1, A2-2, A2-3, A2-4, and A2-5). The calibration curve was linear over a concentration range of 1-50mg/L, which covered the clinically accepted trough and therapeutic plasma levels. The intra- and inter-day precision and accuracy values were both less than 15%. This validated method was successfully applied to therapeutic drug monitoring of teicoplanin in routine clinical practice. Thus, we expect it to be useful for the determination of teicoplanin concentration in human plasma.
建立并验证了一种基于直接进样的、简单、准确且稳健的液相色谱-串联质谱法（LC-MS/MS），用于测定人血浆中的替考拉宁。患者血浆样品在注入LC-MS/MS系统之前，先用缓冲水溶液进行稀释。使用Cadenza HS-C18柱，以乙腈-水（均含有0.1%甲酸）的梯度混合物作为流动相，流速为0.5mL/min，实现色谱分离。在多反应监测模式下，采用正离子电喷雾电离检测分析物。替考拉宁的浓度以六种组分（A3-1、A2-1、A2-2、A2-3、A2-4和A2-5）的总和来确定。校准曲线在1-50mg/L的浓度范围内呈线性，涵盖了临床认可的谷浓度和治疗血浆水平。日内和日间间间精密度和准确度值均小于15%。该验证方法已成功应用于常规临床实践中替考拉宁的治疗药物监测。因此，我们期望它可用于测定人血浆中替考拉宁的浓度。
