Peng Xue-Qian, Zhou Hui-Fen, Lu Yi-Yu, Chen Jun-Kui, Wan Hai-Tong, Zhang Yu-Yan
Zhejiang Chinese Medical University, Hangzhou 310053, China.
Center for Disease Control and Prevention of Zhejiang Province, Hangzhou 310009, China.
Int Immunopharmacol. 2016 Jan;30:85-93. doi: 10.1016/j.intimp.2015.11.029. Epub 2015 Dec 3.
Yinhuapinggan granule (YHPG), a Chinese medicine granule on the basis of Ma-Huang-Tang (Ephedra Decoction) and the clinical experience of Professor Wan Haitong, has been shown to inhibit the growth of influenza virus in vitro. The aim of this study was to investigate the protective effects of YHPG on mice with influenza viral pneumonia and its effects on regulating related inflammatory cytokines in influenza virus A-infected mice. ICR mice were inoculated intranasally with 15 LD50 viral dose of influenza virus A/PR/8/34 (H1N1) and treatments with YHPG (7.5, 15 and 30g/kg) were orally administrated daily for 5 consecutive days after challenge, respectively. The results showed that mortality rate, lung index, lung histopathological changes, IL-6 and TNF-α in serum were significantly attenuated in the treatment of YHPG (15 and 30g/kg) than those in the IFV control group, while the levels of IL-2 was significantly enhanced. Moreover, the RT-PCR results revealed that YHPG (15 and 30g/kg) significantly depressed the expressions of IL-1β, IFN-γ and TNF-α mRNA in lung tissues. Furthermore, the immunohistochemical staining results also revealed that the expression of NF-κB p65 proteins was downregulated when treated with YHPG (15 and 30g/kg). These results showed YHPG has protective effects on IFV-infected mice, due to its ability of alleviation of lung damage, regulation of the cytokine production via inhibiting the NF-κB p65 activation, attenuation of systemic and pulmonary inflammatory responses.
银花平感颗粒（YHPG）是一种基于麻黄汤并结合万海同教授临床经验研制的中药颗粒，已被证明在体外能抑制流感病毒的生长。本研究旨在探讨YHPG对流感病毒性肺炎小鼠的保护作用及其对甲型流感病毒感染小鼠相关炎性细胞因子的调节作用。将15 LD50病毒剂量的甲型流感病毒A/PR/8/34（H1N1）经鼻接种于ICR小鼠，攻毒后分别以YHPG（7.5、15和30 g/kg）连续5天每日灌胃给药。结果显示，与流感病毒（IFV）对照组相比，YHPG（15和30 g/kg）治疗组小鼠的死亡率、肺指数、肺组织病理学变化、血清白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）均显著减轻，而白细胞介素-2（IL-2）水平显著升高。此外，逆转录-聚合酶链反应（RT-PCR）结果显示，YHPG（15和30 g/kg）显著降低了肺组织中IL-1β、干扰素-γ（IFN-γ）和TNF-α mRNA的表达。此外，免疫组织化学染色结果还显示，YHPG（15和30 g/kg）处理后核因子-κB p65（NF-κB p65）蛋白的表达下调。这些结果表明，YHPG对IFV感染小鼠具有保护作用，其机制可能是减轻肺损伤、通过抑制NF-κB p65激活调节细胞因子产生、减轻全身和肺部炎症反应。
