Araujo Ricardo, Caramalho Rita, Coutinho Carla, Sá-Correia Isabel
IPATIMUP, Institute of Molecular Pathology and Immunology, University of Porto, Porto, Portugal Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal
IPATIMUP, Institute of Molecular Pathology and Immunology, University of Porto, Porto, Portugal Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal Division of Hygiene and Medical Microbiology, Innsbruck Medical University, Innsbruck, Austria.
J Clin Microbiol. 2016 Feb;54(2):483-8. doi: 10.1128/JCM.02476-15. Epub 2015 Dec 9.
We propose an optimized protocol for an extensive population analysis of Burkholderia cepacia and Burkholderia contaminans. Seven new polymorphisms were added to the recently proposed SNaPBcen assay, and a total of 18 markers ensured the clear identification and distinction of B. cepacia and B. contaminans isolates and high genotypic discrimination (Simpson index of 0.94) compared to those for multilocus sequence typing.
我们提出了一种优化方案,用于对洋葱伯克霍尔德菌和污染伯克霍尔德菌进行广泛的群体分析。在最近提出的SNaPBcen检测方法中增加了7个新的多态性位点,总共18个标记确保了洋葱伯克霍尔德菌和污染伯克霍尔德菌分离株的清晰鉴定和区分,与多位点序列分型相比具有较高的基因型鉴别力(辛普森指数为0.94)。