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J Clin Microbiol. 2010 May;48(5):1888-91. doi: 10.1128/JCM.02359-09. Epub 2010 Feb 24.

2

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本文引用的文献

1

RecA gene sequence and Multilocus Sequence Typing for species-level resolution of Burkholderia cepacia complex isolates.用于洋葱伯克霍尔德菌复合体分离株种水平鉴定的RecA基因序列和多位点序列分型

Lett Appl Microbiol. 2009 Nov;49(5):580-8. doi: 10.1111/j.1472-765X.2009.02709.x. Epub 2009 Aug 7.

2

Cross-sectional and longitudinal multilocus sequence typing of pseudomonas aeruginosa in cystic fibrosis sputum samples.肺囊性纤维化患者痰液中铜绿假单胞菌的横断面和纵向多位点序列分型。

J Clin Microbiol. 2009 Nov;47(11):3444-8. doi: 10.1128/JCM.00459-09. Epub 2009 Aug 26.

3

Expanded multilocus sequence typing for burkholderia species.伯克霍尔德菌属的多位点序列分型扩展

J Clin Microbiol. 2009 Aug;47(8):2607-10. doi: 10.1128/JCM.00770-09. Epub 2009 Jun 3.

4

Taxon K, a complex within the Burkholderia cepacia complex, comprises at least two novel species, Burkholderia contaminans sp. nov. and Burkholderia lata sp. nov.分类单元K是洋葱伯克霍尔德菌复合群中的一个复合体，包含至少两个新物种，即污染伯克霍尔德菌新种和宽伯克霍尔德菌新种。

Int J Syst Evol Microbiol. 2009 Jan;59(Pt 1):102-11. doi: 10.1099/ijs.0.001123-0.

5

Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex.迟缓伯克霍尔德菌新种、扩散伯克霍尔德菌新种、树栖伯克霍尔德菌新种、种子伯克霍尔德菌新种和金属伯克霍尔德菌新种，伯克霍尔德菌洋葱伯克霍尔德菌复合群内的新物种。

Int J Syst Evol Microbiol. 2008 Jul;58(Pt 7):1580-90. doi: 10.1099/ijs.0.65634-0.

6

Diversity of the parB and repA genes of the Burkholderia cepacia complex and their utility for rapid identification of Burkholderia cenocepacia.洋葱伯克霍尔德菌复合体parB和repA基因的多样性及其在快速鉴定新型洋葱伯克霍尔德菌中的应用。

BMC Microbiol. 2008 Mar 7;8:44. doi: 10.1186/1471-2180-8-44.

7

Multilocus sequence typing breathes life into a microbial metagenome.多位点序列分型为宏基因组注入活力。

PLoS One. 2006 Dec 20;1(1):e17. doi: 10.1371/journal.pone.0000017.

8

Evidence of coinfection with distinct strains of Burkholderia multivorans in a cystic fibrosis patient.一名囊性纤维化患者感染不同菌株的多食伯克霍尔德菌的共感染证据。

Infection. 2006 Oct;34(5):289-91. doi: 10.1007/s15010-006-5608-4.

9

Multilocus sequence typing scheme that provides both species and strain differentiation for the Burkholderia cepacia complex.多位点序列分型方案可对洋葱伯克霍尔德菌复合体进行菌种和菌株鉴别。

J Clin Microbiol. 2005 Sep;43(9):4665-73. doi: 10.1128/JCM.43.9.4665-4673.2005.

10

The multifarious, multireplicon Burkholderia cepacia complex.多样的、多复制子洋葱伯克霍尔德菌复合体

Nat Rev Microbiol. 2005 Feb;3(2):144-56. doi: 10.1038/nrmicro1085.

直接非培养法对囊性纤维化患者痰液样本中的洋葱伯克霍尔德菌复合群进行种系型分析。

Direct culture-independent Strain typing of Burkholderia cepacia complex in sputum samples from patients with cystic fibrosis.

机构信息

Department of Pediatrics, 2nd Medical School, Charles University, V Uvalu 84, 150 06 Prague, Czech Republic.

出版信息

J Clin Microbiol. 2010 May;48(5):1888-91. doi: 10.1128/JCM.02359-09. Epub 2010 Feb 24.

DOI:10.1128/JCM.02359-09

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2863923/

Abstract

We examined if multilocus sequence typing (MLST), a method for genotyping and species identification of Burkholderia cepacia complex bacteria, could be applied directly to cystic fibrosis sputum. The redesigned nested-PCR MLST format was successfully used to accurately identify strains in 23 sputum samples, of which 8 were culture negative.

摘要

我们研究了多基因序列分型（MLST）方法是否可以直接应用于囊性纤维化痰液中的洋葱伯克霍尔德氏菌复合细菌的基因分型和物种鉴定。经过重新设计的巢式 PCR MLST 格式成功地用于准确鉴定 23 个痰液样本中的菌株，其中 8 个样本的培养结果为阴性。