直接非培养法对囊性纤维化患者痰液样本中的洋葱伯克霍尔德菌复合群进行种系型分析。
Direct culture-independent Strain typing of Burkholderia cepacia complex in sputum samples from patients with cystic fibrosis.
机构信息
Department of Pediatrics, 2nd Medical School, Charles University, V Uvalu 84, 150 06 Prague, Czech Republic.
出版信息
J Clin Microbiol. 2010 May;48(5):1888-91. doi: 10.1128/JCM.02359-09. Epub 2010 Feb 24.
Abstract
We examined if multilocus sequence typing (MLST), a method for genotyping and species identification of Burkholderia cepacia complex bacteria, could be applied directly to cystic fibrosis sputum. The redesigned nested-PCR MLST format was successfully used to accurately identify strains in 23 sputum samples, of which 8 were culture negative.
摘要
我们研究了多基因序列分型（MLST）方法是否可以直接应用于囊性纤维化痰液中的洋葱伯克霍尔德氏菌复合细菌的基因分型和物种鉴定。经过重新设计的巢式 PCR MLST 格式成功地用于准确鉴定 23 个痰液样本中的菌株，其中 8 个样本的培养结果为阴性。
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