Aki Shiori Sugamata, Umeda Masaaki
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0192, Japan.
JST, CREST, Ikoma, Nara, 630-0192, Japan.
Methods Mol Biol. 2016;1370:51-7. doi: 10.1007/978-1-4939-3142-2_4.
This chapter focuses on visualization of cell cycle progression in plant tissues using the dual-color marker system "Cytrap." The Cytrap line carries a part of Arabidopsis CDT1a fused to the red fluorescent protein (RFP) gene, which monitors the cell cycle phases from S to late G2 or early mitosis, and the G2/M-specific cyclin B1 marker fused to the green fluorescent protein (GFP) gene. We introduce growth conditions of Arabidopsis roots, the setup for microscopic observation, and analysis of obtained images.
本章重点介绍使用双色标记系统“Cytrap”对植物组织中的细胞周期进程进行可视化。Cytrap株系携带与红色荧光蛋白（RFP）基因融合的拟南芥CDT1a的一部分，其监测从S期到G2晚期或有丝分裂早期的细胞周期阶段，以及与绿色荧光蛋白（GFP）基因融合的G2/M特异性细胞周期蛋白B1标记。我们介绍了拟南芥根的生长条件、显微镜观察设置以及对所得图像的分析。
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