Fluckiger Ricardo, De Caroli Monica, Piro Gabriella, Dalessandro Giuseppe, Neuhaus Jean-Marc, Di Sansebastiano Gian-Pietro
Laboratorio di Botanica, Di STe BA, Università di Lecce, via pro le Lecce-Monteroni, I-73100 Lecce, Italy.
J Exp Bot. 2003 Jun;54(387):1577-84. doi: 10.1093/jxb/erg160. Epub 2003 Apr 11.
Green fluorescent protein (GFP) allows the direct visualization of gene expression and the subcellular localization of fusion proteins in living cells. The localization of different GFP fusion proteins in the secretory system was studied in stably transformed Arabidopsis plants cv. Wassilewskaja. Secreted GFP (SGFP) and GFP retained in the ER (GFP-KDEL) confirmed patterns already known, but two vacuolar GFPs (GFP-Chi and Aleu-GFP) labelled the Arabidopsis vacuolar system for the first time, the organization of which appears to depend on cell differentiation. GFP stability in the vacuoles may depend on pH or degradation, but these vacuolar markers can, nevertheless, be used as a tool for physiological studies making these plants suitable for mutagenesis and gene-tagging experiments.
绿色荧光蛋白（GFP）能够直接观察活细胞中的基因表达以及融合蛋白的亚细胞定位。在稳定转化的拟南芥cv. Wassilewskaja植株中研究了不同GFP融合蛋白在分泌系统中的定位。分泌型GFP（SGFP）和保留在内质网中的GFP（GFP-KDEL）证实了已知的模式，但两种液泡型GFP（GFP-Chi和Aleu-GFP）首次标记了拟南芥的液泡系统，其组织结构似乎依赖于细胞分化。GFP在液泡中的稳定性可能取决于pH值或降解情况，但这些液泡标记物仍可作为生理研究的工具，使这些植物适用于诱变和基因标签实验。
