用于细胞片层脱离的具有温度响应性聚合物的单轴循环应变细胞培养平台的表面改性。
Surface modification of uniaxial cyclic strain cell culture platform with temperature-responsive polymer for cell sheet detachment.
作者信息
Lee E L, Bendre H H, Kalmykov A, Wong J Y
机构信息
Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA.
Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA ; Department of Materials Science and Engineering, Boston University, Boston, MA 02215, USA.
出版信息
J Mater Chem B. 2015 Oct 28;3(40):7899-7902. doi: 10.1039/c5tb01171j. Epub 2015 Aug 19.
Abstract
Current cell sheet-based blood vessels lack biomimetic structure and require excessively long culture times that may compromise smooth muscle cell phenotype. We modified a commercially available product for uniaxial cell sheet conditioning with thermoresponsive copolymers. Thus, culture of detachable conditioned cell sheets is shortened while retaining structural integrity and contractility.
摘要
目前基于细胞片的血管缺乏仿生结构，并且需要过长的培养时间，这可能会损害平滑肌细胞表型。我们用热响应共聚物对一种市售产品进行了改性，用于单轴细胞片处理。因此，可分离的处理过的细胞片的培养时间缩短，同时保持结构完整性和收缩性。
相似文献
1
Surface modification of uniaxial cyclic strain cell culture platform with temperature-responsive polymer for cell sheet detachment.用于细胞片层脱离的具有温度响应性聚合物的单轴循环应变细胞培养平台的表面改性。
J Mater Chem B. 2015 Oct 28;3(40):7899-7902. doi: 10.1039/c5tb01171j. Epub 2015 Aug 19.
2
In-depth analysis of switchable glycerol based polymeric coatings for cell sheet engineering.用于细胞片工程的可切换甘油基聚合物涂层的深入分析。
Acta Biomater. 2015 Oct;25:43-55. doi: 10.1016/j.actbio.2015.06.036. Epub 2015 Jul 2.
3
Terminally functionalized thermoresponsive polymer brushes for simultaneously promoting cell adhesion and cell sheet harvest.用于同时促进细胞黏附和细胞片收获的端基功能化温敏聚合物刷。
Biomacromolecules. 2012 Jan 9;13(1):253-60. doi: 10.1021/bm201545u. Epub 2011 Dec 6.
4
Intelligent thermoresponsive substrate from modified overhead projection sheet as a tool for construction and support of cell sheets in vitro.改性 overhead projection 片基智能温敏基底作为体外细胞片构建和支撑工具。
Tissue Eng Part C Methods. 2011 Feb;17(2):181-91. doi: 10.1089/ten.TEC.2009.0783. Epub 2010 Nov 9.
5
Effect of the hydrophobic basal layer of thermoresponsive block co-polymer brushes on thermally-induced cell sheet harvest.热响应性嵌段共聚物刷的疏水基层对热诱导细胞片收获的影响。
J Biomater Sci Polym Ed. 2012;23(10):1301-14. doi: 10.1163/092050611X580454. Epub 2012 May 11.
6
Human amniotic cell sheet harvest using a novel temperature-responsive culture surface coated with protein-based polymer.使用涂有蛋白质基聚合物的新型温度响应培养表面收获人羊膜细胞片。
Tissue Eng. 2006 Feb;12(2):391-401. doi: 10.1089/ten.2006.12.391.
7
Transfer of fibroblast sheets cultured on thermoresponsive dishes with membranes.在带有膜的热响应性培养皿上培养的成纤维细胞片的转移。
J Mater Sci Mater Med. 2016 Jun;27(6):111. doi: 10.1007/s10856-016-5718-1. Epub 2016 May 6.
8
Two-dimensional manipulation of cardiac myocyte sheets utilizing temperature-responsive culture dishes augments the pulsatile amplitude.利用温度响应培养皿对心肌细胞片进行二维操作可增强搏动幅度。
Tissue Eng. 2001 Apr;7(2):141-51. doi: 10.1089/107632701300062732.
9
Retrieval of rat aortic smooth muscle cells as intact cell sheet for regenerative medicine: a cost effective approach using photo polymerization.利用光聚合技术从大鼠主动脉平滑肌细胞中提取完整的细胞片用于再生医学：一种具有成本效益的方法。
Biotechnol Lett. 2011 Oct;33(10):2083-9. doi: 10.1007/s10529-011-0652-2. Epub 2011 Jun 10.
10
Functional bioengineered corneal epithelial sheet grafts from corneal stem cells expanded ex vivo on a temperature-responsive cell culture surface.在温度响应性细胞培养表面上体外扩增的角膜干细胞来源的功能性生物工程角膜上皮片移植体。
Transplantation. 2004 Feb 15;77(3):379-85. doi: 10.1097/01.TP.0000110320.45678.30.
引用本文的文献
1
Thermoresponsive Pyrrolidone Block Copolymer Organogels from 3D Micellar Networks.基于3D胶束网络的热响应性吡咯烷酮嵌段共聚物有机凝胶
ACS Omega. 2017 Jan 13;2(1):105-112. doi: 10.1021/acsomega.6b00327. eCollection 2017 Jan 31.
本文引用的文献
1
Induced pluripotent stem (iPS) cells: a new source for cell-based therapeutics?诱导多能干细胞（iPS 细胞）：细胞治疗的新来源？
J Control Release. 2014 Jul 10;185:37-44. doi: 10.1016/j.jconrel.2014.04.011. Epub 2014 Apr 16.
2
Engineering cell alignment in vitro.体外工程细胞定向排列。
Biotechnol Adv. 2014 Mar-Apr;32(2):347-65. doi: 10.1016/j.biotechadv.2013.11.007. Epub 2013 Nov 22.
3
The initiation of blood flow and flow induced events in early vascular development.血流的启动和早期血管发育中的血流诱导事件。
Semin Cell Dev Biol. 2011 Dec;22(9):1028-35. doi: 10.1016/j.semcdb.2011.09.020. Epub 2011 Oct 5.
4
The use of micropatterning to control smooth muscle myosin heavy chain expression and limit the response to transforming growth factor β1 in vascular smooth muscle cells.利用微图案化控制平滑肌肌球蛋白重链表达并限制血管平滑肌细胞对转化生长因子β1 的反应。
Biomaterials. 2011 Jan;32(2):410-8. doi: 10.1016/j.biomaterials.2010.08.105. Epub 2010 Sep 19.
5
Cell culture platform with mechanical conditioning and nondamaging cellular detachment.具有机械调节和无损细胞分离功能的细胞培养平台。
J Biomed Mater Res A. 2010 May;93(2):411-8. doi: 10.1002/jbm.a.32754.
6
Cell sheet engineering: a unique nanotechnology for scaffold-free tissue reconstruction with clinical applications in regenerative medicine.细胞片层工程：一种独特的无支架组织重建纳米技术，在再生医学中有临床应用。
J Intern Med. 2010 Jan;267(1):54-70. doi: 10.1111/j.1365-2796.2009.02185.x.
7
Design of prevascularized three-dimensional cell-dense tissues using a cell sheet stacking manipulation technology.使用细胞片层堆叠操作技术设计预血管化的三维细胞致密组织。
Biomaterials. 2010 Mar;31(7):1646-54. doi: 10.1016/j.biomaterials.2009.11.036. Epub 2009 Dec 3.
8
Cellular control of tissue architectures using a three-dimensional tissue fabrication technique.使用三维组织制造技术对组织结构进行细胞控制。
Biomaterials. 2007 Nov;28(33):4939-46. doi: 10.1016/j.biomaterials.2007.08.002. Epub 2007 Aug 20.
9
Transplantation of 3D scaffolds seeded with human embryonic stem cells: biological features of surrogate tissue and teratoma-forming potential.接种人胚胎干细胞的3D支架移植：替代组织的生物学特性和畸胎瘤形成潜力。
Regen Med. 2007 May;2(3):289-300. doi: 10.2217/17460751.2.3.289.
10
Effects of static and cyclic loading in regulating extracellular matrix synthesis by cardiovascular cells.静态和循环负荷对心血管细胞调节细胞外基质合成的影响。
Cardiovasc Res. 2006 Dec 1;72(3):375-83. doi: 10.1016/j.cardiores.2006.08.017. Epub 2006 Sep 1.
Zotero 插件