用于细胞片层脱离的具有温度响应性聚合物的单轴循环应变细胞培养平台的表面改性。
Surface modification of uniaxial cyclic strain cell culture platform with temperature-responsive polymer for cell sheet detachment.
作者信息
Lee E L, Bendre H H, Kalmykov A, Wong J Y
机构信息
Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA.
Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA ; Department of Materials Science and Engineering, Boston University, Boston, MA 02215, USA.
出版信息
J Mater Chem B. 2015 Oct 28;3(40):7899-7902. doi: 10.1039/c5tb01171j. Epub 2015 Aug 19.
Current cell sheet-based blood vessels lack biomimetic structure and require excessively long culture times that may compromise smooth muscle cell phenotype. We modified a commercially available product for uniaxial cell sheet conditioning with thermoresponsive copolymers. Thus, culture of detachable conditioned cell sheets is shortened while retaining structural integrity and contractility.
目前基于细胞片的血管缺乏仿生结构，并且需要过长的培养时间，这可能会损害平滑肌细胞表型。我们用热响应共聚物对一种市售产品进行了改性，用于单轴细胞片处理。因此，可分离的处理过的细胞片的培养时间缩短，同时保持结构完整性和收缩性。
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